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Our Abpromise guarantee covers the use of ab42497 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19470799|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 94 kDa.|
|IHC-P||Use a concentration of 3 µg/ml.
Western Blot: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
ab42497 (3µg/ml) staining of paraffin embedded Human Kidney. The tissue sections were subjected to antigen retrieval by microwave in Tris/EDTA buffer. The HRP-staining procedure was used for detection.
Ab42497 staining TTC10 in HepG2 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Samples were incubated with primary antibody at 10µg/ml for 1 hour. An Alexa Fluor® 488 secondary antibody was used at 2 µg/ml. DAPI was used as a nuclear counterstain. An unimmunized goat IgG (10 µg/ml) was used for negative control.
ab42497 staining TTC10 in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton ×100. Samples were incubated with primary antibody (1/100:in DMEM w/ 10% FBS) for 1 hour at 200C. An Alexa Fluor ® 546-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.
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