概述

  • 产品名称Anti-TTBK1抗体
    参阅全部 TTBK1 一抗
  • 描述
    兔多克隆抗体to TTBK1
  • 经测试应用适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 240 - 270 of Human TTBK1

  • 阳性对照
    • WB: Mouse testis tissue lysates IHC-P: brain tissue IF/ICC: SKNSH cell line.

性能

应用

Our Abpromise guarantee covers the use of ab103944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 143 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU.
  • 组织特异性Expressed in the brain, particularly in cortical and hippocampal neurons. Weakly expressed in spinal cord and testis. No expression in adipose tissue, bladder, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus, thyroid or trachea.
  • 序列相似性Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BDTK antibody
    • Brain derived tau kinase antibody
    • Brain-derived tau kinase antibody
    • KIAA1855 antibody
    • Tau tubulin kinase 1 antibody
    • Tau-tubulin kinase 1 antibody
    • TTBK1 antibody
    • TTBK1_HUMAN antibody
    see all

Anti-TTBK1 antibody 图像

  • ab103944 stained SKNSH cells. The cells were 4% formalehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103944 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-TTBK1 antibody (ab103944) at 1/100 dilution + Mouse testis tissue lysates at 35 µg

    Predicted band size : 143 kDa
  • ab103944, at a 1/50 dilution, staining TTBK1 in formalin fixed and paraffin embedded brain tissue, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Anti-TTBK1 antibody (ab103944)参考文献

ab103944 has not yet been referenced specifically in any publications.

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