概述

  • 产品名称Anti-TSPAN3抗体
    参阅全部 TSPAN3 一抗
  • 描述
    兔多克隆抗体to TSPAN3
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region of Human TSPAN3 (NP_944492)

  • 阳性对照
    • Hela cell lysate.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab81018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 28 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent dilution.

靶标

  • 相关性TSPAN3 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene. Two alternative transcripts encoding different isoforms have been described.
  • 细胞定位Membrane
  • 数据库链接
  • 别名
    • Tetraspan 3 antibody
    • Tetraspan TM4SF antibody
    • tetraspanin 3 antibody
    • tetraspanin TM4 A antibody
    • TM4 A antibody
    • TM4A antibody
    • TM4SF8 antibody
    • transmembrane 4 superfamily member 8 antibody
    • TSPAN 3 antibody
    • TSPAN3 antibody
    see all

Anti-TSPAN3 antibody 图像

  • Anti-TSPAN3 antibody (ab81018) at 1 µg/ml + Hela cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab81018 stained Hek293 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81018, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • All lanes : Anti-TSPAN3 antibody (ab81018) at 1 µg/ml

    Lane 1 : non-reduced GST–tetraspanin EC2 expressed in E. coli
    Lane 2 : reduced GST–tetraspanin EC2 expressed in E. coli

    Lysates/proteins at 1 µg per lane.


    Predicted band size : 28 kDa

Anti-TSPAN3 antibody (ab81018)参考文献

ab81018 has not yet been referenced specifically in any publications.

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