概述

  • 产品名称Anti-TSLP抗体
    参阅全部 TSLP 一抗
  • 描述
    兔多克隆抗体to TSLP
  • 经测试应用适用于: IHC-P, ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length TSLP protein (Human),>98% pure

性能

  • 形式Lyophilised:Reconstitute with 100ul of sterile water to 1mg/ml.
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: None
    Constituents: 0.5xPBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was purified by affinity chromatography employing immobilized hTSLP matrix.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab47943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 0.125 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
ELISA Use a concentration of 0.5 - 2 µg/ml. This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

靶标

  • 功能Cytokine that induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.
  • 组织特异性Expressed in a number of tissues including heart, liver and prostate.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Thymic stromal lymphopoietin antibody
    • Thymic stromal lymphopoietin protein TSLP antibody
    • Tslp antibody
    • TSLP protein antibody
    • TSLP_HUMAN antibody
    see all

Anti-TSLP antibody 图像

  • ab47943  staining TSLP in human prostate tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.

  • Anti-TSLP antibody (ab47943) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa,51 kDa,53 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to TSLP.
  • ICC/IF image of ab47943 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab47943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TSLP antibody (ab47943)参考文献

This product has been referenced in:
  • Li L  et al. Thymic Stromal Lymphopoietin Promotes Fibrosis and Activates Mitogen-Activated Protein Kinases in MRC-5 Cells. Med Sci Monit 22:2357-62 (2016). WB ; Human . Read more (PubMed: 27385084) »
  • Bjerkan L  et al. The short form of TSLP is constitutively translated in human keratinocytes and has characteristics of an antimicrobial peptide. Mucosal Immunol N/A:N/A (2014). WB, IHC-P . Read more (PubMed: 24850429) »

See all 4 Publications for this product

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I have checked with the lab and I can confirm that both ab84247 and ab47943 have been prepared in exactly the same way. They have both been produced from sera of rabbits pre-immunized with highly pure (>98%) r...

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