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Our Abpromise guarantee covers the use of ab47943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.125 µg/ml.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|ELISA||Use a concentration of 0.5 - 2 µg/ml. This allows the detection of 0.2 - 0.4 ng/well of recombinant human TSLP, using 100µl/well antibody solution.|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 18 kDa). Detection limit for recombinant hTSLP is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
ab47943 staining TSLP in human prostate tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded sections). Tissue underwent heat mediated antigen retrieval in sodium citrate buffer (pH 6.0). The primary antibody was used at 0.125 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a DAB chromogen.