重组Anti-TSH beta抗体[EPR8198] (ab155958)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8198] to TSH beta
- Suitable for: mIHC, WB, IHC-P
- Reacts with: Rabbit, Human
Related conjugates and formulations
概述
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产品名称
Anti-TSH beta抗体[EPR8198]
参阅全部 TSH beta 一抗 -
描述
兔单克隆抗体[EPR8198] to TSH beta -
宿主
Rabbit -
经测试应用
适用于: mIHC, WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Rabbit, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Rabbit pituitary tissue lysate, Human recombinant TSH alpha/beta protein IHC/P: Human pituitary tissue
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8198 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155958于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
mIHC |
1/500.
|
|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
|
IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
说明 |
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mIHC
1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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相关性
Various hormones are secreted from the anterior pituitary during development and growth. Thyroid Stimulating Hormone (TSH) is secreted from the anterior pituitary and controls thyroid structure and metabolism -
细胞定位
Secreted -
数据库链接
- Entrez Gene: 7252 Human
- Entrez Gene: 100302414 Rabbit
- Omim: 188540 Human
- SwissProt: P01222 Human
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别名
- CHNG4 antibody
- Thyroid stimulating hormone beta subunit antibody
- Thyroid stimulating hormone, beta precursor antibody
see all
图片
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Fluorescence multiplex immunohistochemical analysis of human pituitary gland tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-ACTH stained on corticotrophs (ab233954; green; Opal™520) at 1:5000 (0.214 μg/ml) [Panel B], anti-TSH beta stained on thyrotrophs (ab155958; red; Opal™570) at 1:500 (1.728 μg/ml) [Panel C], and anti-Growth Hormone stained on somatotrophs (ab155276; gray; Opal™690) at 1:5000 (0.044 μg/ml) [Panel D] on human pituitary gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of ab155276, ab233954, and ab155958 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.
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Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution + Rabbit pituitary tissue lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution + Human recombinant TSH alpha/beta protein at 0.01 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
Immunohistochemical analysis of paraffin-embedded Human pituitary tissue, labeling TSH beta with ab155958 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab155958 被引用在 2 文献中.
- Qiao N et al. Low-rank fusion convolutional neural network for prediction of remission after stereotactic radiosurgery in patients with acromegaly: a proof-of-concept study. J Pathol 258:49-57 (2022). PubMed: 35657600
- Zhang S et al. Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development. Nat Commun 11:5275 (2020). PubMed: 33077725