Anti-TSGA14抗体(ab86180)
Key features and details
- Rabbit polyclonal to TSGA14
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TSGA14抗体 -
描述
兔多克隆抗体to TSGA14 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Gorilla, Orangutan -
免疫原
A region between residue 150 and 200 of human TSGA14 (NP_061188.1).
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阳性对照
- HeLa and 293T whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Affinity purified using an epitope specific to TSGA14 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab86180于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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WB
1/2000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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功能
Required during ciliogenesis for tubulin glutamylation in cilium. Probably acts by participating to the transport of TTLL6, a tubulin polyglutamylase, between the basal body and the cilium. -
组织特异性
Isoform 1 and isoform 4 are expressed in testis and fetal tissues. -
疾病相关
Defects in CEP41 are the cause of Joubert syndrome type 15 (JBTS15) [MIM:614464]. JBTS15 is an autosomal recessive disorder presenting with cerebellar ataxia, oculomotor apraxia, hypotonia, neonatal breathing abnormalities and psychomotor delay. Neuroradiologically, it is characterized by cerebellar vermian hypoplasia/aplasia, thickened and reoriented superior cerebellar peduncles, and an abnormally large interpeduncular fossa, giving the appearance of a molar tooth on transaxial slices (molar tooth sign). Additional variable features include retinal dystrophy, renal disease, liver fibrosis and polydactyly.
Note=Genetic variations in CEP41 may be associated with susceptibility to autism. -
序列相似性
Belongs to the CEP41 family.
Contains 1 rhodanese domain. -
结构域
Although it contains a rhodanese domain, does not display phosphatase activity, suggesting that the protein is enzymatically inactive (PubMed:22246503). -
细胞定位
Cytoplasm > cytoskeleton > centrosome. Cell projection > cilium. Cytoplasm > cytoskeleton > cilium basal body. Localizes mainly to the cilium basal body and in primary cilia. - Information by UniProt
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数据库链接
- Entrez Gene: 95681 Human
- Omim: 610523 Human
- SwissProt: Q9BYV8 Human
- Unigene: 368315 Human
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别名
- centrosomal protein 41 kDa antibody
- Centrosomal protein of 41 kDa antibody
- CEP 41 antibody
see all
图片
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All lanes : Anti-TSGA14 antibody (ab86180) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg/ml
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa
Additional bands at: 100 kDa, 200 kDa, 250 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of Human TSGA14 by Immunoprecipitation, using ab86180 at 3µg/mg lysate. Image shows immunoprecipitated TSGA14 detected with post IP WB, loading 20% of IP and using HeLa whole cell lysate at 1mg. Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab86180 尚未被引用在任何文献中。