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Fusion protein containing amino acids 167-374 of the TSG101 protein expressed in E. coli.
This product was changed from ascites to tissue culture supernatant on 12th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab83 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. PubMed: 23045692|
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/100 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).|
|IHC-P||1/100. Do not perform antigen retrieval.|
Immunohistochemical analysis of paraffin-embedded colon carcinoma tissue labeling TSG101 with ab83 at 1/100 dilution.
Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.
Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1:400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.
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