Anti-TSEN34抗体(ab68868)
Key features and details
- Mouse polyclonal to TSEN34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TSEN34抗体
参阅全部 TSEN34 一抗 -
描述
小鼠多克隆抗体to TSEN34 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human TSEN34. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- TSEN34 transfected 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituents: 0.2% Monobasic dihydrogen potassium phosphate, 0.6% Dibasic monohydrogen sodium phosphate, 8% Sodium chloride, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68868于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
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说明 |
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WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa). |
靶标
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功能
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. -
疾病相关
Pontocerebellar hypoplasia 2C -
序列相似性
Belongs to the tRNA-intron endonuclease family. -
细胞定位
Nucleus. Nucleus, nucleolus. May be transiently localized in the nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 79042 Human
- Omim: 608754 Human
- SwissProt: Q9BSV6 Human
- Unigene: 15580 Human
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别名
- HsSen34 antibody
- LENG 5 antibody
- LENG5 antibody
see all
图片
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All lanes : Anti-TSEN34 antibody (ab68868) at 1/500 dilution
Lane 1 : TSEN34 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (1)
ab68868 被引用在 1 文献中.
- Breuss MW et al. Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly. Am J Hum Genet 99:228-35 (2016). PubMed: 27392077