Synthetic peptide within a region containing residues IDSAVKMLVS LKMSYKAAAG EDYKADCPPG NPAPTSNHGP DATEAEEDFV , corresponding to N terminal amino acids 36-85 of Human Tryptophanyl tRNA synthetase (NP_004175)
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
功能Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
序列相似性Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.
翻译后修饰Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.