The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
1/50 - 1/1000.
Use at an assay dependent concentration.
功能Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
序列相似性Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.
翻译后修饰Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
Norris EL et al. Proteoform-Specific Insights into Cellular Proteome Regulation. Mol Cell Proteomics15:3297-3320 (2016).
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Al-Gubory KH et al. Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy. Reproduction147:599-614 (2014).
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