Anti-Tryptophan Hydroxylase (phospho S260)抗体(ab30574)


  • 产品名称
    Anti-Tryptophan Hydroxylase (phospho S260)抗体
    参阅全部 Tryptophan Hydroxylase 一抗
  • 描述
    兔多克隆抗体to Tryptophan Hydroxylase (phospho S260)
  • 特异性
    Ab30574 recognises Tryptophan Hydroxylase (phospho S260).
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Synthetic phosphopeptide corresponding to amino acid residues surrounding the phospho Ser260 of rat Tryptophan Hydroxylase.

  • 阳性对照
    • Rat brain stem lysate, human dorsal Raphe nucleus.



Our Abpromise guarantee covers the use of ab30574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).


  • 组织特异性
    Isoform 2 seems to be less widely expressed than isoform 1.
  • 通路
    Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • 序列相似性
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Indoleacetic acid 5 hydroxylase antibody
    • L tryptophan hydroxylase antibody
    • MGC119994 antibody
    • TPH 1 antibody
    • TPH antibody
    • TPH1 antibody
    • TPH1_HUMAN antibody
    • TPRH antibody
    • TRPH antibody
    • Tryptophan 5 hydroxylase 1 antibody
    • Tryptophan 5 monooxygenase 1 antibody
    • Tryptophan 5 monooxygenase antibody
    • Tryptophan 5-hydroxylase 1 antibody
    • Tryptophan 5-monooxygenase 1 antibody
    • Tryptophan hydroxylase 1 antibody
    see all

Anti-Tryptophan Hydroxylase (phospho S260) antibody 图像

  • All lanes : Anti-Tryptophan Hydroxylase (phospho S260) antibody (ab30574) at 1/1000 dilution

    Lane 1 : rat brain stem lysate (control)
    Lane 2 : rat brain stem lysate, incubated with S260 phosphopeptide (negative control)
    Lane 3 : rat brain stem lysate, incubated with corresponding non phosphopeptide.

    Lysates/proteins at 15 µg per lane.

    Predicted band size : 51 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 48 kDa,59 kDa,<43 kDa. We are unsure as to the identity of these extra bands.


Anti-Tryptophan Hydroxylase (phospho S260) antibody (ab30574)参考文献

This product has been referenced in:
  • Damen R  et al. The stimulatory adenosine receptor ADORA2B regulates serotonin (5-HT) synthesis and release in oxygen-depleted EC cells in inflammatory bowel disease. PLoS One 8:e62607 (2013). Read more (PubMed: 23638125) »
  • Chin A  et al. The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion. Am J Physiol Gastrointest Liver Physiol 302:G397-405 (2012). WB ; Human . Read more (PubMed: 22038827) »

See all 3 Publications for this product

Product Wall

The sequence of the antigen for this antibody is conserved only in TPH1, however it may still recognize TPH2 although there is not 100 % homology with the TPH2 sequence. The ability of this antibody to recognize TPH2 specifically has not yet been dire...

Read More

Thank you for your enquiry. I have been in touch with the source of this antibody and I can confirm that the lysate used for that blot was made from untreated rat brain. I hope this information helps, please do not hesitate to contact us if you ...

Read More

This antibody is specific for TPH1. Please contact us again if you have any additional questions.