Anti-Tryptophan Hydroxylase抗体(ab46200)

概述

  • 产品名称Anti-Tryptophan Hydroxylase抗体
    参阅全部 Tryptophan Hydroxylase 一抗
  • 描述
    兔多克隆抗体to Tryptophan Hydroxylase
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat
    预测可用于: Rabbit
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Tryptophan Hydroxylase.

    (Peptide available as ab46755.)

  • 阳性对照
    • This antibody gave a positive signal in Mouse Brain Tissue Lysate.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab46200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 组织特异性Isoform 2 seems to be less widely expressed than isoform 1.
  • 通路Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • 序列相似性Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Indoleacetic acid 5 hydroxylase antibody
    • L tryptophan hydroxylase antibody
    • MGC119994 antibody
    • TPH 1 antibody
    • TPH antibody
    • TPH1 antibody
    • TPH1_HUMAN antibody
    • TPRH antibody
    • TRPH antibody
    • Tryptophan 5 hydroxylase 1 antibody
    • Tryptophan 5 monooxygenase 1 antibody
    • Tryptophan 5 monooxygenase antibody
    • Tryptophan 5-hydroxylase 1 antibody
    • Tryptophan 5-monooxygenase 1 antibody
    • Tryptophan hydroxylase 1 antibody
    see all

Anti-Tryptophan Hydroxylase antibody 图像

  • ICC/IF image of ab46200 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46200, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) PC12 cells at 1µg/ml.
  • Anti-Tryptophan Hydroxylase antibody (ab46200) at 0.004 µg/ml + Mouse Brain Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Tryptophan Hydroxylase antibody (ab46200)参考文献

ab46200 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 04 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Specification Brain
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Jan 04 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"