This product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Conjugation: Use at an assay dependent dilution.
Dot: Use at an assay dependent dilution.
ELISA: 1/3000 - 1/12000. This product has been assayed against 1.0µg of Trypsin Inhibitor [Soybean] in a standard sandwich ELISA using Peroxidase conjugated affinity purified anti-Rabbit IgG and ABTS as a substrate for 30 minutes at room temperature.
WB: Use at an assay dependent dilution. Predicted molecular weight: 25 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
The soybean trypsin inhibitor was first crystallized by Kunitz in 1945 and is one of several such inhibitors found in soybeans. It inhibits the proteolytic action approximately of an equal weight of crystalline trypsin by combining with trypsin to form a stable compound. Chymotrypsin is only slightly inhibited by soy inhibitor.