Anti-TRUB1抗体(ab72866)

概述

  • 产品名称Anti-TRUB1抗体
    参阅全部 TRUB1 一抗
  • 描述
    小鼠多克隆抗体to TRUB1
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human protein (NP_631908.1)

  • 阳性对照
    • WB: mammalian transfected lysate. ICC/IF: HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能May be responsible for synthesis of pseudouridine from uracil in transfer RNAs.
  • 组织特异性Highly expressed in heart, skeletal muscle and liver. Expressed at lower levels in lung, small intestine, kidney and spleen.
  • 序列相似性Belongs to the pseudouridine synthase TruB family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Probable tRNA pseudouridine synthase 1 antibody
    • PUS4 antibody
    • TruB pseudouridine (psi) synthase homolog 1 (E. coli) antibody
    • Trub1 antibody
    • TRUB1_HUMAN antibody
    see all

Anti-TRUB1 antibody 图像

  • All lanes : Anti-TRUB1 antibody (ab72866) at 1/500 dilution

    Lane 1 : TRUB1-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 37 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells using protein A affiinity purified ab72866 TRUB1 at 10 ug/ml.

Anti-TRUB1 antibody (ab72866)参考文献

ab72866 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry regarding ab72866. I can confirm that this antibody is affinity purified and we have just removed the purification note in the application section to avoid any further confusion. It has only been tested for Western bl...

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