• 产品名称Anti-TRPV4抗体
    参阅全部 TRPV4 一抗
  • 描述
    兔多克隆抗体to TRPV4
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Human, Pig
    预测可用于: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 720-769 (


    ) of Human TRPV4 isoform 2 (NP_671737)

  • 阳性对照
    • HT1080 cell lysate



Our Abpromise guarantee covers the use of ab94868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


  • 功能Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
  • 组织特异性Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
  • 疾病相关Brachyolmia 3
    Spondylometaphyseal dysplasia Kozlowski type
    Metatropic dysplasia
    Distal spinal muscular atrophy, congenital non-progressive
    Charcot-Marie-Tooth disease 2C
    Scapuloperoneal spinal muscular atrophy
    Spondyloepiphyseal dysplasia Maroteaux type
    Parastremmatic dwarfism
    Digital arthropathy-brachydactyly, familial
  • 序列相似性Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
    Contains 3 ANK repeats.
  • 翻译后修饰Phosphorylation results in enhancement of its channel function.
  • 细胞定位Cell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCYM3 antibody
    • CMT2C antibody
    • HMSN2C antibody
    • osm 9 like TRP channel 4 antibody
    • Osm-9-like TRP channel 4 antibody
    • OSM9 like transient receptor potential channel 4 antibody
    • Osmosensitive transient receptor potential channel 4 antibody
    • OTRPC 4 antibody
    • OTRPC4 antibody
    • SMAL antibody
    • SPSMA antibody
    • SSQTL1 antibody
    • Transient receptor potential cation channel subfamily V member 4 antibody
    • Transient receptor potential protein 12 antibody
    • TRP 12 antibody
    • TRP12 antibody
    • TRPV 4 antibody
    • TrpV4 antibody
    • TRPV4_HUMAN antibody
    • Vanilloid receptor like channel 2 antibody
    • Vanilloid receptor like protein 2 antibody
    • Vanilloid receptor related osmotically activated channel antibody
    • Vanilloid receptor-like channel 2 antibody
    • Vanilloid receptor-like protein 2 antibody
    • Vanilloid receptor-related osmotically-activated channel antibody
    • VR 4 antibody
    • VR OAC antibody
    • VR-OAC antibody
    • VR4 antibody
    • VRL 2 antibody
    • VRL-2 antibody
    • VRL2 antibody
    • VROAC antibody
    see all

Anti-TRPV4 antibody 图像

  • Predicted band size : 91 kDa
    Western blot analysis of PANC1 cells labeling TRPV4 with ab94868 at 1.0µg/ml.
  • ab94868 staining TRPV4 in cultured hippocampal slices of rat brain by Immunohistochemistry - PFA fixed sections.

    Slice cultures attached to the PTFE membrane were fixed with 4% paraformaldehyde in PBS for 20 minutes at room temperature. They were permeabilized with 1% Triton X-100 in PBS overnight at 4 °C, then blocked with 3% bovine serum albumin (BSA) in PBS and incubated overnight at 4 °C with ab94868 (Red) at a 1/500 dilution. Following washes with PBS and blocking with 3% BSA, tissue was incubated for 1 hour at room temperature with Alexa Fluor 568-conjugated goat anti-rabbit antibody diluted 1/500 in blocking solution. Tissue was washed in PBS, mounted with DAPI.

    Blue: DAPI.
    Green: anti-Map2.
    Red: ab94868.
  • Anti-TRPV4 antibody (ab94868) at 1 µg/ml + HT1080 cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 91 kDa
    Gel concentration: 6-18%

Anti-TRPV4 antibody (ab94868)参考文献

ab94868 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (Bio-Rad 4–20% Mini-PROTEAN­ TGX™ Gel, 12 well, 20 ´l 456-1095)
Sample Pig Tissue lysate - whole (coronary artery)
Specification coronary artery
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

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提交于 Aug 29 2014