概述

  • 产品名称Anti-TRPV3抗体
    参阅全部 TRPV3 一抗
  • 描述
    兔多克隆抗体to TRPV3
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human TRPV3.

    (Peptide available as ab32733.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: U-87 MG; SK N BE; SHSY-5Y; HeLa This antibody gave a positive result in IHC in the following FFPE tissue: Human normal hippocampus.

性能

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应用

Our Abpromise guarantee covers the use of ab32734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IHC-P
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 90 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity.
    • 组织特异性Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level).
    • 序列相似性Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV3 sub-subfamily.
      Contains 3 ANK repeats.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 1110036I10Rik antibody
      • MGC124324 antibody
      • MGC124325 antibody
      • Transient receptor potential cation channel subfamily V member 3 antibody
      • TrpV3 antibody
      • Trpv3 heat sensitive channel antibody
      • TRPV3_HUMAN antibody
      • Vanilloid receptor 3 antibody
      • Vanilloid receptor like 3 antibody
      • Vanilloid receptor-like 3 antibody
      • vanilloid receptor-related osmotically activated channel protein antibody
      • VRL 3 antibody
      • VRL-3 antibody
      • VRL3 antibody
      see all

    Anti-TRPV3 antibody 图像

    • IHC image of TRPV3 staining in Human normal hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32734, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • All lanes : Anti-TRPV3 antibody (ab32734) at 1 µg/ml

      Lane 1 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
      Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
      Lane 3 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
      Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 90 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)


      Exposure time : 20 minutes

    Anti-TRPV3 antibody (ab32734)参考文献

    ab32734 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"