Anti-TRPS1抗体(ab125197)
Key features and details
- Rabbit polyclonal to TRPS1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TRPS1抗体
参阅全部 TRPS1 一抗 -
描述
兔多克隆抗体to TRPS1 -
宿主
Rabbit -
经测试应用
适用于: WB, IP, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1000-1050 of Human TRPS1 (AAG21134.1).
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阳性对照
- WB: Jurkat, 293T, HeLa whole cell lysate. IP: HeLa cells. IHC-P: Human breast carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125197于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/2000 - 1/10000. Predicted molecular weight: 142 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000.
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说明 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 142 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. |
靶标
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功能
Transcriptional repressor. Binds specifically to GATA sequences and represses expression of GATA-regulated genes at selected sites and stages in vertebrate development. Regulates chondrocyte proliferation and differentiation. Executes multiple functions in proliferating chondrocytes, expanding the region of distal chondrocytes, activating proliferation in columnar cells and supporting the differentiation of columnar into hypertrophic chondrocytes. -
组织特异性
Ubiquitously expressed in the adult. Found in fetal brain, lung, kidney, liver, spleen and thymus. More highly expressed in androgen-dependent than in androgen-independent prostate cancer cells. -
疾病相关
Defects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 1 (TRPS1) [MIM:190350]. TRPS1 is an autosomal dominant disorder characterized by craniofacial and skeletal abnormalities. It is allelic with tricho-rhino-phalangeal type 3. Typical features include sparse scalp hair, a bulbous tip of the nose, protruding ears, a long flat philtrum and a thin upper vermilion border. Skeletal defects include cone-shaped epiphyses at the phalanges, hip malformations and short stature.
Defects in TRPS1 are a cause of tricho-rhino-phalangeal syndrome type 2 (TRPS2) [MIM:150230]. A syndrome that combines the clinical features of trichorhinophalangeal syndrome type 1 and multiple exostoses type 1. Affected individuals manifest multiple dysmorphic facial features including large, laterally protruding ears, a bulbous nose, an elongated upper lip, as well as sparse scalp hair, winged scapulae, multiple cartilaginous exostoses, redundant skin, and mental retardation. Note=A chromosomal aberration resulting in the loss of functional copies of TRPS1 and EXT1 has been found in TRPS2 patients.
Defects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 3 (TRPS3) [MIM:190351]. TRPS3 is an autosomal dominant disorder characterized by craniofacial and skeletal abnormalities. It is allelic with tricho-rhino-phalangeal type 1. In TRPS3 a more severe brachydactyly and growth retardation are observed. -
序列相似性
Contains 7 C2H2-type zinc fingers.
Contains 1 GATA-type zinc finger. -
翻译后修饰
Sumoylated. Sumoylation in the repressor domain inhibits the transcription repression activity. Sumoylation on Lys-1201 is the major site. Appears to be sumoylated on multiple sites. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 537652 Cow
- Entrez Gene: 7227 Human
- Entrez Gene: 299897 Rat
- Omim: 604386 Human
- SwissProt: Q9UHF7 Human
- Unigene: 657018 Human
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别名
- GC79 antibody
- LGCR antibody
- Transcriptional repressor GATA binding 1 antibody
see all
图片
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All lanes : Anti-TRPS1 antibody (ab125197) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 142 kDa
Exposure time: 3 minutes -
ab125197 at 1 µg/ml staining TRPS1 by WB, following immunoprecipitation of whole cell lysate from HeLa cells.
Lane 1; IP using ab125197 at 6 µg/mg lysate.
Lane 2; IP using control IgG.
1 mg of lysate was used for IP and 20% of IP was loaded. Detection utilised Chemiluminescence with a 30 second exposure. -
Paraffin embedded human breast carcinoma tissue stained for TRPS1 using ab125197 at 1/1000 dilution in immunohistochemical analysis.
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All lanes : Anti-TRPS1 antibody (ab125197) at 1/500 dilution
Lane 1 : Ha-Ras-transformed EpH4 cells - nuclear extracts
Lanes 2-3 : EpF-2 and EpF-1 (EpH4 subclones expressing high amounts of Zeb1 and Zeb2 mRNA) - nuclear extracts
Lane 4 : EpH4 (parental) - nuclear extracts
Lane 5 : EpF-2 - nuclear extracts
Lanes 6-7 : EpF-Z1.1 and EpF-Z1.2 (EpF-1 clones transfected with siRNA to knockdown Zeb1) - nuclear extracts
Lanes 8-9 : EpF-Z2.1 and EpF-Z2.2 (EpF-1 clones transfected with siRNA to knockdown Zeb2) - nuclear extracts
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP-conjugated donkey anti-rabbit IgG polyclonal at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 142 kDa
Exposure time: 20 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab125197 被引用在 1 文献中.
- Zhao T et al. Paclitaxel Resistance Modulated by the Interaction between TRPS1 and AF178030.2 in Triple-Negative Breast Cancer. Evid Based Complement Alternat Med 2022:6019975 (2022). PubMed: 35399640