概述

  • 产品名称Anti-TRPM8抗体
    参阅全部 TRPM8 一抗
  • 描述
    兔多克隆抗体to TRPM8
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rabbit, Dog, Monkey, Baboon
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human TRPM8.

    (Peptide available as ab95862.)

  • 阳性对照
    • This antibody gave a positive signal in the following human whole cell lysates: HeLa; PC3.

性能

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应用

Our Abpromise guarantee covers the use of ab85617 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 127 kDa (predicted molecular weight: 127 kDa).

靶标

  • 功能Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • 组织特异性Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • 序列相似性Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Long transient receptor potential channel 6 antibody
    • LTrpC-6 antibody
    • LTrpC6 antibody
    • MGC2849 antibody
    • Short form of the TRPM8 cationic channel antibody
    • Transient receptor potential cation channel subfamily M member 8 antibody
    • Transient receptor potential p8 antibody
    • transient receptor potential-p8 antibody
    • Trp p8 antibody
    • Trp-p8 antibody
    • Trpm8 antibody
    • TRPM8_HUMAN antibody
    • TRPP8 antibody
    see all

Anti-TRPM8 antibody 图像

  • All lanes : Anti-TRPM8 antibody (ab85617) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 127 kDa
    Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,87 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-TRPM8 antibody (ab85617)参考文献

This product has been referenced in:
  • Bidaux G  et al. Regulation of Activity of Transient Receptor Potential Melastatin 8 (TRPM8) Channel by Its Short Isoforms. J Biol Chem 287:2948-62 (2012). WB ; Human . Read more (PubMed: 22128173) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (stably human TRPM8-transfected HEK293 cells)
Total protein in input 500 µg
Specification stably human TRPM8-transfected HEK293 cells
Immuno-precipitation step Protein A
Username

Miss. Larisa Maier

Verified customer

提交于 May 15 2013

Merci beaucoup aussi pourcet émail etpour votre retour sur cet anticorps.

Je suis désolée quecet anticorpsne fonctionne pas dans les autres applications. Comme nous ne l'avions pas testé, nous ne pouvions pas non p...

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Je suis désolée d'apprendre que ab85617 ne vous donne pas de bons résultats en ni en Western blot ni en IF.

Nous aimerons bien investiguer ceci. Veuillez trouver ci-joint un questionnaire qui nous permet de regrouper le plus d'informations ...

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