Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
Immunohistochemistry (Frozen sections) - Anti-TRPM8 antibody (ab3243)Image courtesy of an anonymous Abreview.
ab3243 staining TRPM8 in murine skin tissue by Immunohistochemistry (Frozen sections). Tissue was fixed in methanol, permeabilized with 0.4% Triton X-100, blocked using 10% serum for 30 minutes at 25°C and then incubated with ab3243 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.
Liu T et al. RNA interference-mediated depletion of TRPM8 enhances the efficacy of epirubicin chemotherapy in prostate cancer LNCaP and PC3 cells. Oncol Lett15:4129-4136 (2018).
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