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The clone number has been updated from S74-25 to N74/25, both clone numbers name the same antibody clone.
Our Abpromise guarantee covers the use of ab85016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 212 kDa. Prepare membrane fraction and ensure proteolysis is avoided. After extraction use an SDS buffer, incubate for 15 mins at 37°C before running on SDS-Page.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ab85016 staining TRPM7 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"