概述

  • 产品名称Anti-TRPM7抗体
    参阅全部 TRPM7 一抗
  • 描述
    山羊多克隆抗体to TRPM7
  • 经测试应用适用于: IHC-P, IHC-Fr, ELISA, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Crayfish
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide: TKESESTNSVRLML, corresponding to C terminal amino acids 1852-1865 of Human TRPM7.

  • 阳性对照
    • Daudi lysate
  • 常规说明

    In response to customer feedback and in-house testing the latest lot doesn't pass the WB quality testing so we have decided to remove the application for now. Please contact technical@abcam.com for more details.

     

    Please see reference He et al for details of the size of the protein detected by SDS-PAGE. Principal Names: TRPM7; transient receptor potential cation channel, subfamily M, member 7; CHAK; CHAK1; LTRPC7; FLJ20117; TRP-PLIK; LTRPC ion channel family member 7; homolog of mouse transient receptor potential-phospholipase C-interacting kinase CHaK hypothetical protein. Official Gene Symbol - TRPM7 GenBank Accession Number – XP_030709 LocusLink ID - 54822 (human).

     Can be blocked with Human TRPM7 peptide (ab22777).

     

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration. PubMed: 21183474
IHC-Fr 1/1000. PubMed: 16285951
ELISA 1/16000.
ICC/IF Use at an assay dependent concentration. PubMed: 16285951
IHC-FoFr Use at an assay dependent concentration. PubMed: 19734892

靶标

  • 功能Essential ion channel and serine/threonine-protein kinase. Divalent cation channel permeable to calcium and magnesium. Has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. The kinase activity is essential for the channel function. May be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell. Phosphorylates annexin A1 (ANXA1).
  • 疾病相关Defects in TRPM7 are a cause of susceptibility to amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 (ALS-PDC1) [MIM:105500]; also called amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam or Guam disease. Amyotrophic lateral sclerosis-parkinsonism/dementia complex type 1 is a neurodegenerative disorder characterized by chronic, progressive and uniformly fatal amyotrophic lateral sclerosis and parkinsonism-dementia. Both diseases are known to occur in the same kindred, the same sibship and even the same individual.
  • 序列相似性In the C-terminal section; belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
    In the N-terminal section; belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM7 sub-subfamily.
    Contains 1 alpha-type protein kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CHAK antibody
    • CHAK1 antibody
    • Channel kinase 1 antibody
    • Channel-kinase 1 antibody
    • Long transient receptor potential channel 7 antibody
    • LTrpC-7 antibody
    • LTrpC7 antibody
    • Transient receptor potential cation channel subfamily M member 7 antibody
    • TRP PLIK antibody
    • TRPM7 antibody
    • TRPM7_HUMAN antibody
    see all

Anti-TRPM7 antibody (ab729)参考文献

This product has been referenced in:
  • Inoue H  et al. Mg(2+)- and ATP-dependent inhibition of transient receptor potential melastatin 7 by oxidative stress. Free Radic Biol Med 72:257-66 (2014). WB ; Human . Read more (PubMed: 24747489) »
  • Sun H  et al. Role of TRPM7 Channels in Hyperglycemia-Mediated Injury of Vascular Endothelial Cells. PLoS One 8:e79540 (2013). WB ; Human . Read more (PubMed: 24223965) »

See all 9 Publications for this product

Product Wall

Could you send us the following information;

- lot number
- purchase order number

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and would like to investigate the complaint further. Could you send us the publication o...

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Thank you for your reply.


For anti-TRPM7 the immunogen region for ab729 and ab72155 show 100% homology with the bovine sequence. Therefore, either of these antibodies should work with bovine.

http://www.uniprot.org/uniprot/F1MP...

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Thank you for contacting us.

The ab let me know the following:
The observed molecular weight corresponds to earlier findings in the literature with different antibodies. We are not sure if this applies also to TRPM6.

I hope t...

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Thank you for contacting us.

I am checking with the lab regarding ab729 and will let you know.

As for TRPM6, which antibody are you refering to?

I look forward to hear back from you.

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Thank you for your reply. The details provided enable us to closely monitor the quality of our products.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have iss...

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Thank you for your email.

I can definitely send you 2 replacement antibodies to the address you mentioned, but I would need to know which ones you'd like to receive.

I can send the same antibodies and different lots if we have them...

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Thank you for your reply.

I have located both your orders for ab729 and ab85016.

Could you please let me know if you would prefer a free of charge replacement for each of the antibodies, a credit or refund? We do carry other TRPM7 ...

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Thank you for your recent participation in our survey.
I am very sorry to hear that the protocol tips did not work.

You had used ab729 as well as ab85016.What tips have you tried and have the results changed at all?

Please let ...

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Thank you for contacting us. I have received WB protocol information for ab729, but am still waiting for more information regarding ab85016. Thank you for your continued patience in the meantime. For ab729, we have seen a 150-160kDa band in DDI, ...

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