Anti-TRPM3抗体(ab56171)

概述

  • 产品名称
    Anti-TRPM3抗体
  • 描述
    兔多克隆抗体to TRPM3
  • 经测试应用
    适用于: IHC-Fr, ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    A region within synthetic peptide: CKLCKAMAHE ASENDMVDDI SQELNHNSRD FGQLAVELLD QSYKQDEQLA, corresponding to internal sequence amino acids 541-590 of Human TRPM3

  • 阳性对照
    • HepG2 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab56171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr 1/1000.
ELISA 1/312500. This antibody has been tested against the recombinant peptide.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 198 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 相关性
    Transient receptor potential cation channel, subfamily M, member 3 (TRPM3) belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation selective channels important for cellular calcium signaling and homeostasis. TRPM3 mediates calcium entry, and this entry is potentiated by calcium store depletion. Its activity is increased by reduction in extracellular osmolarity, by store depletion and muscarinic receptor activation. The TRPM3-mediated calcium entry is inhibited by the nonselective calcium channel blocker lanthanide gadolinium. Expressed primarily in the kidney and, at lower levels, in brain, testis, ovary, pancreas and spinal cord. In the kidney, expressed predominantly in the collecting tubular epithelium in the medulla, medullary rays, and periglomerular regions; in the brain, highest levels are found in the cerebellum, choroid plexus, the locus coeruleus, the posterior thalamus and the substantia nigra. Down-regulated in renal tumors compared to normal kidney. Alternatively spliced transcript variants encoding different isoforms have been identified.
  • 细胞定位
    Membrane; multi pass membrane protein.
  • 数据库链接
  • 别名
    • GON2 antibody
    • long transient receptor potential channel 3 antibody
    • LTrpC 3 antibody
    • LTRPC3 antibody
    • melastatin 2 antibody
    • Melastatin2 antibody
    • MLSN2 antibody
    • transient receptor potential cation channel, subfamily M, member 3 antibody
    see all

图片

  • Anti-TRPM3 antibody (ab56171) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 198 kDa
  • ab56171 staining TRPM3 in Rat murine choroid plexus cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in PFA and permeabilized in Triton X-100 prior to blocking in 0.5% BSA in TBS-Tween for 20 minutes at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Cy3®-conjugated Goat anti-Rabbit IgG (H+L) polyclonal, diluted 1/400. Nuclei counterstained with DAPI.

    See Abreview

文献

ab56171 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Spinal cord)
Permeabilization
Yes - 0.1% Triton X in PBS
Specification
Spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Feb 25 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0,1% Triton X-100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Jul 20 2011

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human glioblastoma cell line)
Specification
human glioblastoma cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - Tritonx100 0.1% in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 29 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (human glioblastoma cell lines)
Loading amount
100 µg
Specification
human glioblastoma cell lines
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 23 2010

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (murine choroid plexus cell line)
Specification
murine choroid plexus cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - Tritonx100 0.1% in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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