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A region within synthetic peptide: CKLCKAMAHE ASENDMVDDI SQELNHNSRD FGQLAVELLD QSYKQDEQLA, corresponding to internal sequence amino acids 541-590 of Human TRPM3
Our Abpromise guarantee covers the use of ab56171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||1/312500. This antibody has been tested against the recombinant peptide.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 198 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
ab56171 staining TRPM3 in Rat murine choroid plexus cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in PFA and permeabilized in Triton X-100 prior to blocking in 0.5% BSA in TBS-Tween for 20 minutes at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Cy3®-conjugated Goat anti-Rabbit IgG (H+L) polyclonal, diluted 1/400. Nuclei counterstained with DAPI.
ab56171 has not yet been referenced specifically in any publications.
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