概述

  • 产品名称Anti-TRPM2抗体
    参阅全部 TRPM2 一抗
  • 描述
    兔多克隆抗体to TRPM2
  • 经测试应用适用于: IHC-P, IHC-FoFr, ICC, WB, IPmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, which represents a portion of the human transient receptor potential cation channel, subfamily M, member 2 encoded within exon 13 (LocusLink ID 7226).

性能

应用

Our Abpromise guarantee covers the use of ab11168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
ICC 1/50.
WB 1/300. Predicted molecular weight: 171 kDa.
IP Use at 1-4 µg/mg of lysate.

靶标

  • 功能Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Isoform 2 does not seem to be regulated by ADPR. Has ADP-ribose pyrophosphatase activity.
  • 组织特异性Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.
  • 序列相似性Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.
    Contains 1 nudix hydrolase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EREG 1 antibody
    • EREG1 antibody
    • Estrogen responsive element associated gene 1 antibody
    • Estrogen responsive element associated gene 1 protein antibody
    • Estrogen-responsive element-associated gene 1 protein antibody
    • KNP 3 antibody
    • KNP3 antibody
    • Long transient receptor potential channel 2 antibody
    • LTRPC 2 antibody
    • LTrpC-2 antibody
    • LTrpC2 antibody
    • MGC133383 antibody
    • NUDT9H antibody
    • NUDT9L1 antibody
    • putative Ca2+ channel protein antibody
    • putative Ca2+ channel protein antibody
    • Transient receptor potential cation channel subfamily M member 2 antibody
    • Transient receptor potential channel 7 antibody
    • TRPC 7 antibody
    • TrpC7 antibody
    • TRPM 2 antibody
    • Trpm2 antibody
    • TRPM2_HUMAN antibody
    see all

Anti-TRPM2 antibody 图像

  • IHC image of ab11168 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11168, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-TRPM2 antibody (ab11168)参考文献

This product has been referenced in:
  • Ahn C  et al. Regulation and Localization of Transient Receptor Potential Melastatin 2 in Rat Uterus. Reprod Sci N/A:N/A (2014). Rat . Read more (PubMed: 24577155) »
  • Lee CR  et al. TRPM2 channels are required for NMDA-induced burst firing and contribute to H(2)O(2)-dependent modulation in substantia nigra pars reticulata GABAergic neurons. J Neurosci 33:1157-68 (2013). Read more (PubMed: 23325252) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Neutrophil)
Permeabilization No
Specification Neutrophil
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Sep 25 2015

Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching ou...

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Thank you for your enquiry. There appears to be three human isoforms of this target (Swiss Prot). TRPM2-S should have a predicted KDa of 101 and TRPM2-L should have a predicted KDa of 180 (Swiss Prot).I have removed the predicted KDa of 17 from the dat...

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Thank you for your enquiry. There appears to be three human isoforms of this target (Swiss Prot). TRPM2-S should have a predicted KDa of 101 and TRPM2-L should have a predicted KDa of 180 (Swiss Prot).I have removed the predicted KDa of 17 from the dat...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"