Anti-TRPC4抗体[S77-15] (ab84813)

概述

  • 产品名称Anti-TRPC4抗体[S77-15]
    参阅全部 TRPC4 一抗
  • 描述
    小鼠单克隆抗体[S77-15] to TRPC4
  • 经测试应用适用于: WB, IP, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Horse, Guinea pig, Cow, Dog, Pig, Macaque Monkey
  • 免疫原

    Synthetic peptide:

    FKSEKVVVEDTVPIIPKE

    , corresponding to C terminal amino acids 930-947 of Rat TRPC4 (accession number O35119)

  • 阳性对照
    • COS cell lysate transiently transfected with TRPC4

性能

应用

Our Abpromise guarantee covers the use of ab84813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 110 kDa.
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 0.1 - 1 µg/ml. (Perox), 1 - 10 µg/ml (IF).
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Form a receptor-activated non-selective calcium permeant cation channel. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Mediates cation entry, with an enhanced permeability to barium over calcium. May also be activated by intracellular calcium store depletion.
  • 组织特异性Strongly expressed in placenta. Expressed at lower levels in heart, pancreas, kidney and brain. Expressed in endothelial cells. Isoform alpha was found to be the predominant isoform. Isoform beta was not found in pancreas and brain.
  • 序列相似性Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
    Contains 4 ANK repeats.
  • 结构域The protein 4.1-binding domain (654-685) is required for binding to EPB41L2 and channel activation.
    The calmodulin- and inositol 1,4,5-trisphosphate receptor-binding (CIRB) domain (695-724) is sufficient for the interaction with SESTD1.
    The spectrin-binding domain (730-758) is required for binding to SPTAN1 and SPTBN5.
  • 翻译后修饰Phosphorylation modulates TRPC channel function by regulating the level of TRPC4 at the cell surface and by increasing the association with SLC9A3R1/NHERF.
  • 细胞定位Membrane. Cell membrane. Enhanced insertion into the cell membrane after activation of the EGF receptor.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CCE1 antibody
    • HTRP 4 antibody
    • hTrp-4 antibody
    • hTrp4 antibody
    • MGC119570 antibody
    • MGC119571 antibody
    • MGC119572 antibody
    • MGC119573 antibody
    • RP11-10M8.1 antibody
    • Short transient receptor potential channel 4 antibody
    • STRPC4 antibody
    • Transient receptor potential 4 antibody
    • transient receptor potential cation channel subfamily C antibody
    • Transient receptor potential cation channel subfamily C member 4 antibody
    • transient receptor potential channel 4 antibody
    • TRP 4 antibody
    • trp related protein 4 antibody
    • Trp-related protein 4 antibody
    • TRP4 antibody
    • TRPC 4 antibody
    • TrpC4 antibody
    • TRPC4 protein antibody
    • TRPC4_HUMAN antibody
    see all

Anti-TRPC4 antibody [S77-15] 图像

  • ab84813 staining TRPC4 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.

  • Overlay histogram showing SH-SY5Y cells stained with ab84813 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84813, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-TRPC4 antibody [S77-15] (ab84813)参考文献

ab84813 has not yet been referenced specifically in any publications.

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