重组
RabMAb

Anti-TRPC1抗体[EP1417Y] (ab51255)

概述

  • 产品名称
    Anti-TRPC1抗体[EP1417Y]
    参阅全部 TRPC1 一抗
  • 描述
    兔单克隆抗体[EP1417Y] to TRPC1
  • 经测试应用
    适用于: WB, IP, Flow Cytmore details
    不适用于: ICC/IF or IHC
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Synthetic peptide:

    KFHDFADRKDWDAFC

    (Human)

  • 阳性对照
    • HepG2 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab51255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
IP 1/100.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for ICC/IF or IHC.
  • 靶标

    • 功能
      Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
    • 组织特异性
      Seems to be ubiquitous.
    • 序列相似性
      Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
      Contains 4 ANK repeats.
    • 翻译后修饰
      Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • HTRP 1 antibody
      • HTRP1 antibody
      • MGC133334 antibody
      • MGC133335 antibody
      • Mtrp1 antibody
      • Short transient receptor potential channel 1 antibody
      • Transient receptor potential canonical 1 antibody
      • Transient receptor potential cation channel subfamily C member 1 antibody
      • Transient receptor potential channel 1 antibody
      • Transient receptor protein 1 antibody
      • TRP 1 antibody
      • TRP 1 protein antibody
      • Trp related protein 1 antibody
      • TRP-1 antibody
      • TRP1 antibody
      • TRP1 protein antibody
      • TRPC1 antibody
      • Trpc1 transient receptor potential cation channel subfamily C member 1 antibody
      • TRPC1_HUMAN antibody
      • Trrp1 antibody
      see all

    Anti-TRPC1 antibody [EP1417Y] 图像

    • Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/10000 dilution + HepG2 cell lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 91 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/500 dilution (for 16 hours) + Rat artery tissue lysate at 50 µg

      Secondary
      An HRP-conjugated Goat anti-rabbit polyclonal at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 91 kDa
      Observed band size : ~100 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes

      This image is courtesy of an anonymous Abreview

      See Abreview

    Anti-TRPC1 antibody [EP1417Y] (ab51255)参考文献

    This product has been referenced in:
    • Mazzotta C  et al. Proangiogenic effects of soluble a-Klotho on systemic sclerosis dermal microvascular endothelial cells. Arthritis Res Ther 19:27 (2017). Read more (PubMed: 28183357) »
    • Tajeddine N & Gailly P TRPC1 protein channel is major regulator of epidermal growth factor receptor signaling. J Biol Chem 287:16146-57 (2012). WB ; Human . Read more (PubMed: 22451676) »

    See all 6 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (artery)
    Loading amount
    50 µg
    Specification
    artery
    Gel Running Conditions
    Reduced Denaturing (7.5)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    提交于 Sep 07 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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