概述

  • 产品名称Anti-TRPA1抗体
    参阅全部 TRPA1 一抗
  • 描述
    兔多克隆抗体to TRPA1
  • 经测试应用适用于: IP, IHC-Fr, IHC-FoFr, IHC-P, ICC/IF, IHC - Wholemountmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Human
  • 免疫原

    Synthetic peptide:

    CEKQHELIKLIIQKME

    , corresponding to amino acids 1060-1075 of Rat TRPA1

  • 常规说明We have mixed feedback about mouse specificity from our customers which is why we no longer support this species.

性能

应用

Our Abpromise guarantee covers the use of ab58844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at an assay dependent concentration. PubMed: 25023795
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 21750223
IHC-P Use at an assay dependent concentration. PubMed: 24358160
ICC/IF Use at an assay dependent concentration. PubMed: 21558163
IHC - Wholemount Use at an assay dependent concentration. PubMed: 19632231

靶标

  • 功能Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
  • 组织特异性Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.
  • 序列相似性Belongs to the transient receptor (TC 1.A.4) family.
    Contains 15 ANK repeats.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANKTM 1 antibody
    • ANKTM1 antibody
    • Ankyrin-like with transmembrane domains protein 1 antibody
    • Transformation sensitive protein p120 antibody
    • Transformation-sensitive protein p120 antibody
    • Transient receptor potential cation channel subfamily A member 1 antibody
    • TRPA 1 antibody
    • Trpa1 antibody
    • TRPA1_HUMAN antibody
    see all

Anti-TRPA1 antibody 图像

  • TRPA1 staining of rat DRG using FITC-conjugated donkey anti-rabbit secondary antibodies (green color) and propidium iodide as a counterstain.
  • Immunohistochemical analysis of murine distal colon tissue, staining TRPA1 with ab58844.

    Sections were incubated with primary antibody (1/1000) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody. ab36001 was used to stain CGRP (red).
  • Immunohistochemical analysis of wholemount murine colonic wall, staining TRPA1 with ab58844.

    Wholemount preparations were incubated with primary antibody (1/1000) overnight at 37°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.

Anti-TRPA1 antibody (ab58844)参考文献

This product has been referenced in:
  • Urata K  et al. Involvement of TRPV1 and TRPA1 in incisional intraoral and extraoral pain. J Dent Res 94:446-54 (2015). IHC (PFA fixed) ; Rat . Read more (PubMed: 25576470) »
  • Eberhardt M  et al. H2S and NO cooperatively regulate vascular tone by activating a neuroendocrine HNO-TRPA1-CGRP signalling pathway. Nat Commun 5:4381 (2014). IP, IHC-FoFr ; Rat . Read more (PubMed: 25023795) »

See all 9 Publications for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Dorsal root ganglia, all spinal levels)
Loading amount 30 µg
Specification Dorsal root ganglia, all spinal levels
Gel Running Conditions Reduced Denaturing (8% acrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Andrew Grant

Verified customer

提交于 Nov 05 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for your enquiry.

I can confirm thatab58844 Anti-TRPA1 antibodyis sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated o...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry ab62053, sent as free of charge replacement of ab58844, did not perform as stated on the...

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Thank you for your email.

Yes, we do haveone other antibody against TRPA1 which has been tested in frozen sections and on human and mouse tissue - it is the ab62053. http://www.abcam.com/index.html?datasheet=62053 (or use the following: http:...

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Thank you for contacting us.

We have two reports from customers who used the antibody ab58844 for western blotting. In both cases, the antibody was incubated with the membrane at a dilution of 1/1000.

The sizes of the bands detect...

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Thank you for your reply.
I apologize, but I misunderstood your original email and had assumed that you were having trouble seeing bands in WB. I didn't realize you were asking about the expected MW.
Anyway, this antibody has been shown by cust...

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Thank you for contacting us.

Among the antibodies mentioned in your email against TRPA1, we have other alternatives that have indeed been tested in rat species. Please see below the link to their datasheets and verify whether they are suitabl...

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Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (nasopharyngeal carcinoma (NPC) cells)
Specification nasopharyngeal carcinoma (NPC) cells
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Apr 12 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"