Anti-TRPA1抗体(ab56458)

概述

  • 产品名称Anti-TRPA1抗体
    参阅全部 TRPA1 一抗
  • 描述
    小鼠单克隆抗体to TRPA1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: IPNADKSLEM EILKQKYRLK DLTFLLEKQH ELIKLIIQKM EIISETEDDD SHCSFQDRFK KEQMEQRNSR WNTVLRAVKA KTHHL, corresponding to amino acids 1033-1118 of Human TRPA1

性能

应用

Our Abpromise guarantee covers the use of ab56458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
    • 组织特异性Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.
    • 序列相似性Belongs to the transient receptor (TC 1.A.4) family.
      Contains 15 ANK repeats.
    • 细胞定位Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • ANKTM 1 antibody
      • ANKTM1 antibody
      • Ankyrin-like with transmembrane domains protein 1 antibody
      • Transformation sensitive protein p120 antibody
      • Transformation-sensitive protein p120 antibody
      • Transient receptor potential cation channel subfamily A member 1 antibody
      • TRPA 1 antibody
      • Trpa1 antibody
      • TRPA1_HUMAN antibody
      see all

    Anti-TRPA1 antibody 图像

    • Western blot against tagged recombinant protein immunogen using ab56458 TRPA1 antibody at 1ug/ml. Predicted band size of immunogen is 35 kDa

    Anti-TRPA1 antibody (ab56458)参考文献

    ab56458 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Dorsal root ganglia, all spinal levels)
    Loading amount 30 µg
    Specification Dorsal root ganglia, all spinal levels
    Gel Running Conditions Reduced Denaturing (8% acrylamide)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Andrew Grant

    Verified customer

    提交于 Nov 05 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"