Anti-TRP2抗体- N-terminal (ab93473)

概述

  • 产品名称Anti-TRP2抗体- N-terminal
    参阅全部 TRP2 一抗
  • 描述
    兔多克隆抗体to TRP2 - N-terminal
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from the N terminal region of Human TRP2 conjugated to KLH (NP_001913.2).

  • 阳性对照
    • A375 cell line lysates

性能

应用

Our Abpromise guarantee covers the use of ab93473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 59 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Involved in regulating eumelanin and phaeomelanin levels.
    • 通路Pigment biosynthesis; melanin biosynthesis.
    • 序列相似性Belongs to the tyrosinase family.
    • 细胞定位Melanosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • L dopachrome tautomerase antibody
      • DCT antibody
      • Dopachrome delta isomerase antibody
      • Dopachrome tautomerase antibody
      • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
      • DT antibody
      • EC 5.3.3.12 antibody
      • L dopachrome Delta isomerase antibody
      • L-dopachrome Delta-isomerase antibody
      • L-dopachrome tautomerase antibody
      • TRP 2 antibody
      • TRP-2 antibody
      • TRP2 antibody
      • Tyrosinase related protein 2 antibody
      • Tyrosinase-related protein 2 antibody
      • TYRP2 antibody
      • TYRP2_HUMAN antibody
      see all

    Anti-TRP2 antibody - N-terminal 图像

    • Anti-TRP2 antibody - N-terminal (ab93473) at 1/100 dilution + A375 cell line lysates at 35 µg

      Predicted band size : 59 kDa
    • Anti-TRP2 antibody - N-terminal (ab93473) at 1/2000 dilution + A375 whole cell lysate at 10000 µg

      Secondary
      HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 59 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

    Anti-TRP2 antibody - N-terminal (ab93473)参考文献

    ab93473 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A375 (melanoma))
    Loading amount 10000 cells
    Specification A375 (melanoma)
    Gel Running Conditions Reduced Denaturing (10% acrylamide)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    提交于 May 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"