Anti-TRP2抗体- N-terminal (ab93473)

概述

  • 产品名称
    Anti-TRP2抗体- N-terminal
    参阅全部 TRP2 一抗
  • 描述
    兔多克隆抗体to TRP2 - N-terminal
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide selected from the N terminal region of Human TRP2 conjugated to KLH (NP_001913.2).

  • 阳性对照
    • A375 cell line lysates

性能

应用

Our Abpromise guarantee covers the use of ab93473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 59 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      Involved in regulating eumelanin and phaeomelanin levels.
    • 通路
      Pigment biosynthesis; melanin biosynthesis.
    • 序列相似性
      Belongs to the tyrosinase family.
    • 细胞定位
      Melanosome membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • L dopachrome tautomerase antibody
      • DCT antibody
      • Dopachrome delta isomerase antibody
      • Dopachrome tautomerase antibody
      • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
      • DT antibody
      • EC 5.3.3.12 antibody
      • L dopachrome Delta isomerase antibody
      • L-dopachrome Delta-isomerase antibody
      • L-dopachrome tautomerase antibody
      • TRP 2 antibody
      • TRP-2 antibody
      • TRP2 antibody
      • Tyrosinase related protein 2 antibody
      • Tyrosinase-related protein 2 antibody
      • TYRP2 antibody
      • TYRP2_HUMAN antibody
      see all

    图片

    • Anti-TRP2 antibody - N-terminal (ab93473) at 1/100 dilution + A375 cell line lysates at 35 µg

      Predicted band size : 59 kDa
    • Anti-TRP2 antibody - N-terminal (ab93473) at 1/2000 dilution + A375 whole cell lysate at 10000 µg

      Secondary
      HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 59 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

    实验方案

    文献

    ab93473 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A375 (melanoma))
    Loading amount
    10000 cells
    Specification
    A375 (melanoma)
    Gel Running Conditions
    Reduced Denaturing (10% acrylamide)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 May 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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