概述

  • 产品名称
  • 描述
    兔多克隆抗体to TRP2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human TRP2.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: K562; HeLa; A431 and A549.

性能

应用

Our Abpromise guarantee covers the use of ab92995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 mg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).

靶标

  • 功能
    Involved in regulating eumelanin and phaeomelanin levels.
  • 通路
    Pigment biosynthesis; melanin biosynthesis.
  • 序列相似性
    Belongs to the tyrosinase family.
  • 细胞定位
    Melanosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • L dopachrome tautomerase antibody
    • DCT antibody
    • Dopachrome delta isomerase antibody
    • Dopachrome tautomerase antibody
    • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
    • DT antibody
    • EC 5.3.3.12 antibody
    • L dopachrome Delta isomerase antibody
    • L-dopachrome Delta-isomerase antibody
    • L-dopachrome tautomerase antibody
    • TRP 2 antibody
    • TRP-2 antibody
    • TRP2 antibody
    • Tyrosinase related protein 2 antibody
    • Tyrosinase-related protein 2 antibody
    • TYRP2 antibody
    • TYRP2_HUMAN antibody
    see all

图片

  • All lanes : Anti-TRP2 antibody (ab92995) at 1 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • All lanes : Anti-TRP2 antibody (ab92995) at 1/1000 dilution

    Lane 1 : Human A375 whole cell lysate with 21% Oxygen
    Lane 2 : Human A375 whole cell lysate with 1% Oxygen

    Lysates/proteins at 10000 cells per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Daniel Tennant

    See Abreview

实验方案

文献

ab92995 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A375 - melanoma cell line)
Loading amount
10000 cells
Specification
A375 - melanoma cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Daniel Tennant

Verified customer

提交于 May 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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