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Synthetic peptide corresponding to Human TRP2 (internal sequence). synthesized peptide derived from internal of human TRP2.
Our Abpromise guarantee covers the use of ab74073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 59 kDa.
TRP2 protein is a single-pass type I membrane protein, these types of proteins known to aggregate when boiled therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70C instead of boiling.
|ICC/IF||1/100 - 1/500.|
ab74073 staining TRP2 in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.