Anti-TRP2抗体(ab103463)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to TRP2
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Dog, Human
  • 免疫原

    KLH conjugated synthetic peptide corresponding to a region within N terminal amino acids 68-98 of Human TRP2.

  • 阳性对照
    • Human skin

性能

应用

Our Abpromise guarantee covers the use of ab103463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 59 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Involved in regulating eumelanin and phaeomelanin levels.
  • 通路
    Pigment biosynthesis; melanin biosynthesis.
  • 序列相似性
    Belongs to the tyrosinase family.
  • 细胞定位
    Melanosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • L dopachrome tautomerase antibody
    • DCT antibody
    • Dopachrome delta isomerase antibody
    • Dopachrome tautomerase antibody
    • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
    • DT antibody
    • EC 5.3.3.12 antibody
    • L dopachrome Delta isomerase antibody
    • L-dopachrome Delta-isomerase antibody
    • L-dopachrome tautomerase antibody
    • TRP 2 antibody
    • TRP-2 antibody
    • TRP2 antibody
    • Tyrosinase related protein 2 antibody
    • Tyrosinase-related protein 2 antibody
    • TYRP2 antibody
    • TYRP2_HUMAN antibody
    see all

图片

  • Anti-TRP2 antibody (ab103463) at 1/100 dilution + A375 cell lysate at 35 µg

    Predicted band size : 59 kDa
  • ab103463, at a 1/50 dilution, staining TRP2 in formalin-fixed, paraffin-embedded breast carcinoma, using a peroxidase conjugated secondary antibody and DAB staining.
  • ab103643 staining TRP2 in Human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Incubation with the primary antibody was followed by preoxidase conjugation of the secondary antibody and DAB staining. TRP2 staining is indicated by arrows.

文献

ab103463 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (1 kidney, 2 liver, 3 heart, 4 positive control)
Specification
1 kidney, 2 liver, 3 heart, 4 positive control
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 13 2014

Thank you for your phone call. I have created the following discount codes for you:


DISCOUNT CODE: xxx for ab83774
DISCOUNT CODE: xxx for ab103463
DISCOUNT CODE: xxx for ab46545

Expiration date: xxx

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (oral melanoma)
Specification
oral melanoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 15 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A375 - melanoma cell line)
Loading amount
10000 cells
Specification
A375 - melanoma cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 31 2012

Thank you for contacting us. I found the dog sequences and the immunogen forantibody ab103463 has 88% homology with dog, so it is likely to work. That would be the antibody I suggest you try for TRP1. As far as TRP1, I would recommend ab83774. It has n...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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