概述

  • 产品名称Anti-TRP1抗体[TA99]
    参阅全部 TRP1 一抗
  • 描述
    小鼠单克隆抗体[TA99] to TRP1
  • 特异性ab3312 is specific for melanomas and does not react with carcinomas and sarcomas.
  • 经测试应用适用于: IHC-Fr, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    SK-MEL-23 Melanoma cell line.

  • 阳性对照
    • In Western Blot, this antibody gave a positive signal in human skin tissue lysate.

性能

应用

Our Abpromise guarantee covers the use of ab3312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19841138

靶标

  • 功能Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
  • 组织特异性Pigment cells.
  • 通路Pigment biosynthesis; melanin biosynthesis.
  • 疾病相关Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
  • 序列相似性Belongs to the tyrosinase family.
  • 细胞定位Melanosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 antibody
    • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
    • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
    • b-PROTEIN antibody
    • CAS2 antibody
    • Catalase B antibody
    • CATB antibody
    • DHICA oxidase antibody
    • Glycoprotein 75 antibody
    • GP75 antibody
    • Melanoma antigen gp75 antibody
    • OCA3 antibody
    • TRP antibody
    • TRP-1 antibody
    • TRP1 antibody
    • Tyrosinase related protein 1 antibody
    • Tyrosinase-related protein 1 antibody
    • TYRP antibody
    • TYRP1 antibody
    • TYRP1_HUMAN antibody
    • TYRRP antibody
    see all

Anti-TRP1 antibody [TA99] 图像

  • Anti-TRP1 antibody [TA99] (ab3312) at 5 µg/ml + Human skin tissue lysate - total protein (ab30166) at 20 µg

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 72 kDa
    Observed band size : 72 kDa
    Additional bands at : 61 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ab3312 staining TRP1 in B16 mouse melanoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in 2% paraformaldehyde for 30 minutes and permeabilized in 0.1% Triton X-100 prior to blocking in 5% goat serum for 1 hour at 22°C. The primary antibody was diluted 1/200 in 5% goat serum/1% BSA in PBS and incubated with the sample for 1 hour at 22°C. The secondary antibody was Alexa Fluor® 568-conjugated Goat anti-Mouse polyclonal, diluted 1/500.

    See Abreview

Anti-TRP1 antibody [TA99] (ab3312)参考文献

This product has been referenced in:
  • van Niel G  et al. The tetraspanin CD63 regulates ESCRT-independent and -dependent endosomal sorting during melanogenesis. Dev Cell 21:708-21 (2011). Human . Read more (PubMed: 21962903) »
  • Strunnikova NV  et al. Transcriptome analysis and molecular signature of human retinal pigment epithelium. Hum Mol Genet 19:2468-86 (2010). WB ; Human . Read more (PubMed: 20360305) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step 0.1% triton, 2.5% goat serum, 2% BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Tissue sections (Human Skin)
Specification Human Skin
Permeabilization Yes - 0.1% tritonx-100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 09 2014

Thank you for contacting us and your interest in our products.

We have not used the anti-TRP1 antibody [TA99] ab3312 in in vivo assays and would therefore not be able to guarantee the results. I would like to point out that the antibody has 0....

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (SK-MEL-28)
Specification SK-MEL-28
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X in PBS
Username

Abcam user community

Verified customer

提交于 Oct 18 2012

Thank you for confirming these details.

We have added a free of charge unit of ab34165 to your order xx. This free of charge unit has been provided to replace the ab3312.

Please wish the customer all the best of success with his res...

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Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vita...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (B16 mouse melanoma cell line)
Specification B16 mouse melanoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. James Harris

Verified customer

提交于 Sep 27 2011

Thank you for your inquiry. Unfortunately, we do not know whether the light chains in these antibodies (ab3312 and ab74073) are lambda (λ) or kappa (κ). This has not been tested. If you require a secondary antibody for these primary a...

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Unfortunately, the exact epitope recognised by this antibody has not been mapped

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"