Anti-TRP1抗体(ab83774)

概述

  • 产品名称Anti-TRP1抗体
    参阅全部 TRP1 一抗
  • 描述
    兔多克隆抗体to TRP1
  • 经测试应用适用于: IHC-P, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal amino acids 396-445 (

    NDPIFVLLHTFTDAVFDEWLRRYNADISTFPLENAPIGHNRQYNMVPFWP

    ) of Human TRP1 (NP_000541).

  • 阳性对照
    • 721_B cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab83774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 61 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized.
  • 组织特异性Pigment cells.
  • 通路Pigment biosynthesis; melanin biosynthesis.
  • 疾病相关Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3) [MIM:203290]; also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair.
  • 序列相似性Belongs to the tyrosinase family.
  • 细胞定位Melanosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 5 antibody
    • 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody
    • 6-dihydroxyindole-2-carboxylic acid oxidase antibody
    • b-PROTEIN antibody
    • CAS2 antibody
    • Catalase B antibody
    • CATB antibody
    • DHICA oxidase antibody
    • Glycoprotein 75 antibody
    • GP75 antibody
    • Melanoma antigen gp75 antibody
    • OCA3 antibody
    • TRP antibody
    • TRP-1 antibody
    • TRP1 antibody
    • Tyrosinase related protein 1 antibody
    • Tyrosinase-related protein 1 antibody
    • TYRP antibody
    • TYRP1 antibody
    • TYRP1_HUMAN antibody
    • TYRRP antibody
    see all

Anti-TRP1 antibody 图像

  • Anti-TRP1 antibody (ab83774) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • ab83774 staining TRP1 in human melanoma MNT-1 cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.1% Triton X-100 and then blocked using 1% BSA for 1 hour at 22°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 594 (red) used at a 1/1000 dilution.

    See Abreview

  • ab83774 at 5 µg/ml staining TRP1 in Human skin by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-TRP1 antibody (ab83774)参考文献

ab83774 has not yet been referenced specifically in any publications.

Product Wall

Western blot

Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (epidermis)
Specification epidermis
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 May 29 2013

Thank you for your phone call. I have created the following discount codes for you:


DISCOUNT CODE: xxx for ab83774
DISCOUNT CODE: xxx for ab103463
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Expiration date: xxx

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Veuillez trouver la SDS en document attaché. Vous pouvez dès maintenant aussi la télécharger depuis la fiche technique si vous souhaitez par le lien à droite, sous le lien "ge...

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Merci de nous avoir contactés.

Les FDS (ou MSDS) sont disponiblesdirectement depuis les fiches techniques de nos produits:
http://www.abcam.com/ab83774
http://www.abcam.com/ab14509

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (melanoma (oral))
Specification melanoma (oral)
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375 melanoma cell line)
Loading amount 10000 cells
Specification A375 melanoma cell line
Gel Running Conditions Reduced Denaturing (10% acrylamide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 May 31 2012

It was nice speaking to you on the phone too!

I am very happy to hear thay you would like to take up my offer of testing ab104864 and ab83774 with your human and mouse samples respectively. I have generated the testing discount codes as below...

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Thank you for contacting us in regards to antibodies ab104864, ab83774 and ab52493.

As discussed over the phone, the western blot presented on the datasheet of Anti-Hyaluronan synthase 1 antibody [3E10] (ab104864) was performed using the reco...

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Thank you very much for your interest in ab83774. To our knowledge, ab83774 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab83774 now, test it in mouse and submit feedback to us in the form of an A...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human melanoma line MNT-1)
Loading amount 20 µg
Specification human melanoma line MNT-1
Treatment control (C) or WIPI1 (W) siRNA
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Ms. Hsiang Ho

Verified customer

提交于 May 05 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"