Anti-Tropomyosin抗体[TM-311] (ab7785)

概述

  • 产品名称Anti-Tropomyosin抗体[TM-311]
    参阅全部 Tropomyosin 一抗
  • 描述
    小鼠单克隆抗体[TM-311] to Tropomyosin
  • 特异性This antibody is specific for the 36K and 39K bands (alpha isoform at 39 kDa and beta isoform at 36 kDa )of chicken gizzard tropomyosin. It is not specific for the alpha form alone. This antibody stains human fibroblast cells, and it cross-reacts with chicken fibroblast and L8 rat muscle cells.
  • 经测试应用适用于: IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Chicken, Human
  • 免疫原

    Purified chicken gizzard tropomyosin.

  • 阳性对照
    • Chicken or human fibroblasts

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 克隆编号TM-311
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
WB 1/100 - 1/200. Detects a band of approximately 49-59 kDa (predicted molecular weight: 33 kDa).

靶标

Anti-Tropomyosin antibody [TM-311] 图像

  • Anti-Tropomyosin antibody [TM-311] (ab7785) at 1/200 dilution + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 33 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

Anti-Tropomyosin antibody [TM-311] (ab7785)参考文献

This product has been referenced in:
  • Ito A  et al. Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation. Sci Rep 4:4781 (2014). WB ; Mouse . Read more (PubMed: 24759171) »
  • Yamashiro S  et al. Differential actin-regulatory activities of Tropomodulin1 and Tropomodulin3 with diverse tropomyosin and actin isoforms. J Biol Chem 289:11616-29 (2014). WB ; Rabbit . Read more (PubMed: 24644292) »

See all 6 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Myometrium)
Loading amount 20 µg
Specification Myometrium
Gel Running Conditions Reduced Denaturing (gel 12%,)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

提交于 Jan 14 2011

Thank you for your phone call. The originator of ab7785 provided me with the following information regarding this antibody. The molecular weight of tropomyosin is 70 kDa. The alpha isomer is 36 kDa and the beta isomer is 35 kDa. In electrophoresis...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"