Anti-Tropomyosin抗体[MAC 141] (ab50567)

概述

  • 产品名称
    Anti-Tropomyosin抗体[MAC 141]
    参阅全部 Tropomyosin 一抗
  • 描述
    大鼠单克隆抗体[MAC 141] to Tropomyosin
  • 特异性
    Antibody reacts with tropomyosin in Lethocerus and Drosophila flight and non-flight muscle and with tropomyosin in flight muscles of all other insect species tested. Antibody reacts with Troponin H (TnH) in Lethocerus flight muscle, the 33 and 34 isoforms of TnH in Drosophila and proteins of TnH molecular weight in flight muscles of other Diptera and Lepidoptera. Antibody also reacts with tropomyosin in muscles of Crustacea and Caenorhabditis (nematode).
  • 经测试应用
    适用于: WB, IHC-Fr, Electron Microscopy, ICC/IF, IHC - Wholemountmore details
  • 种属反应性
    与反应: Drosophila melanogaster, Waterbug
    预测可用于: Caenorhabditis elegans, A wide range of other invertebrates
  • 免疫原

    Troponin-tropomyosin complex purified from Lethocerus indicus (Waterbug) flight muscle.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    MAC 141
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50567 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-Fr Use at an assay dependent concentration. PubMed: 19272319
Electron Microscopy Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC - Wholemount Use at an assay dependent concentration. PubMed: 22841645

靶标

  • 相关性
    Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
  • 细胞定位
    Cytoplasmic
  • 别名
    • Alpha tropomyosin antibody
    • Beta tropomyosin antibody
    • TM30nm antibody
    • TPM1 antibody
    • TPM2 antibody
    • TPM3 antibody
    • Tropomyosin 1 alpha antibody
    • Tropomyosin 1 antibody
    • Tropomyosin 2 antibody
    • Tropomyosin 2 beta antibody
    • Tropomyosin 3 antibody
    see all

图片

  • Immunohistochemical analysis of wholemount Drosohopila melanogaster embryo, staining Tropomyosin with ab50567 at 1/1000 dilution.
  • Immunohistochemical analysis of wholemount Drosohopila melanogaster embryo, staining Tropomyosin with ab50567 at 1/1000 dilution.

文献

This product has been referenced in:
  • Cabezas-Cruz A  et al. Anaplasma phagocytophilum Infection Subverts Carbohydrate Metabolic Pathways in the Tick Vector, Ixodes scapularis. Front Cell Infect Microbiol 7:23 (2017). ICC/IF . Read more (PubMed: 28229048) »
  • Auld AL & Folker ES Nucleus-dependent sarcomere assembly is mediated by the LINC complex. Mol Biol Cell 27:2351-9 (2016). Read more (PubMed: 27307582) »

See all 16 Publications for this product

客户评价及客户问答

Thank you for your enquiry. I have been informed by the source of this product of the following information: " In 1993 Dr Matthew Colloff (Dept Bacteriology, Stobhill General Hospital, Glasgow) said that MAC 141 reacts with prawn tropomyosin. He ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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