Anti-Tropomyosin抗体(ab76640)

概述

  • 产品名称Anti-Tropomyosin抗体
    参阅全部 Tropomyosin 一抗
  • 描述
    小鼠单克隆抗体to Tropomyosin
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性

    预测可用于: Human
  • 免疫原

    Recombinant full length human Tropomyosin protein (AAH11776) with a tag

  • 阳性对照
    • Recombinant full length Tropomyosin protein (immunogen).

性能

应用

Our Abpromise guarantee covers the use of ab76640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
  • 应用说明ELISA: Use at an assay dependent dilution. Detection limit for ab76640 is approximately 3 ng/ml as a capture antibody.

    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

  • 靶标

    • 相关性Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
    • 细胞定位Cytoplasmic
    • 数据库链接
    • 别名
      • Alpha tropomyosin antibody
      • Beta tropomyosin antibody
      • TM30nm antibody
      • TPM1 antibody
      • TPM2 antibody
      • TPM3 antibody
      • Tropomyosin 1 alpha antibody
      • Tropomyosin 1 antibody
      • Tropomyosin 2 antibody
      • Tropomyosin 2 beta antibody
      • Tropomyosin 3 antibody
      see all

    Anti-Tropomyosin antibody 图像

    • Anti-Tropomyosin antibody (ab76640) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 33 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Anti-Tropomyosin antibody (ab76640)参考文献

    ab76640 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"