概述

  • 产品名称
    Anti-Tropomyosin抗体
    参阅全部 Tropomyosin 一抗
  • 描述
    兔多克隆抗体to Tropomyosin
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Chicken
  • 免疫原

    Recombinant full length protein (Chicken).

  • 阳性对照
    • Total tissue extract.
  • 常规说明
    For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 15mM Sodium Azide
    Constituents: Whole serum
  • 纯度
    Whole antiserum
  • 纯化说明
    Product is supplied as liquid whole antiserum. The antiserum has been treated to remove lipoproteins.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab11190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ICC/IF
  • 应用说明
    IF: 1/40 (determined by indirect immunofluorescent labeling of cultured chicken fibroblasts).
    WB: 1/100 (determined by indirect immunoblotting using total tissue extract).

    Rabbit anti-Tropomyosin may be used for visualization of tropomyosin in various tissues and cell cultures. The product reacts best on cultured chicken fibroblasts.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
    • 细胞定位
      Cytoplasmic
    • 数据库链接
    • 别名
      • Alpha tropomyosin antibody
      • Beta tropomyosin antibody
      • TM30nm antibody
      • TPM1 antibody
      • TPM2 antibody
      • TPM3 antibody
      • Tropomyosin 1 alpha antibody
      • Tropomyosin 1 antibody
      • Tropomyosin 2 antibody
      • Tropomyosin 2 beta antibody
      • Tropomyosin 3 antibody
      see all

    文献

    This product has been referenced in:
    • Goins LM & Mullins RD A novel tropomyosin isoform functions at the mitotic spindle and Golgi in Drosophila. Mol Biol Cell 26:2491-504 (2015). Read more (PubMed: 25971803) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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