重组
RabMAb

Anti-Tropomodulin 2抗体[EPR6353] (ab124833)

概述

  • 产品名称
    Anti-Tropomodulin 2抗体[EPR6353]
    参阅全部 Tropomodulin 2 一抗
  • 描述
    兔单克隆抗体[EPR6353] to Tropomodulin 2
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the N-terminus of Human Tropomodulin 2.

  • 阳性对照
    • Fetal brain and SH-SY5Y cell lysates; Human brain tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab124833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • 应用说明
    Is unsuitable for Flow Cyt.
  • 靶标

    • 相关性
      Tropomodulin is the actin-capping protein for the slow growing end of filamentous actin. Tropomodulin 2 is a neuronal specific isoform which is the major binding protein to brain tropomyosin in rat.
    • 细胞定位
      Cytoplasm; cytoskeleton
    • 数据库链接
    • 别名
      • Tropomodulin 2 antibody
      • MGC39481 antibody
      • N Tmod antibody
      • Neuronal tropomodulin antibody
      • NTMOD antibody
      • TMOD2 antibody
      • tropomodulin 2 (neuronal) antibody
      • tropomodulin 2 neuronal antibody
      see all

    图片

    • All lanes : Anti-Tropomodulin 2 antibody [EPR6353] (ab124833) at 1/10000 dilution

      Lane 1 : Fetal brain lysate
      Lane 2 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 40 kDa
      Observed band size : 40 kDa
    • ab124833, at 1/250 dilution, staining Tropomodulin 2 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    ab124833 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Murine Neuroblastome N1E-115)
    Specification
    Murine Neuroblastome N1E-115
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton, 10min
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma)
    Loading amount
    20 µg
    Specification
    N1E-115 mouse neuroblastoma
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 25 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册