概述

  • 产品名称
    Anti-Tropomodulin 2抗体
    参阅全部 Tropomodulin 2 一抗
  • 描述
    小鼠多克隆抗体to Tropomodulin 2
  • 特异性
    ab67407 is reactive against mammalian transfected lysate
  • 经测试应用
    适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-351 of Human Tropomodulin 2

  • 阳性对照
    • Tropomodulin 2 transfected 293T lysate

性能

应用

Our Abpromise guarantee covers the use of ab67407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
WB
  • 应用说明
    WB: 1/500 - 1/1000. Detects a band of approximately 39.6 kDa.
    Detection Antibody Pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Tropomodulin is the actin-capping protein for the slow growing end of filamentous actin. Tropomodulin 2 is a neuronal specific isoform which is the major binding protein to brain tropomyosin in rat.
    • 细胞定位
      Cytoplasm; cytoskeleton
    • 数据库链接
    • 别名
      • Tropomodulin 2 antibody
      • MGC39481 antibody
      • N Tmod antibody
      • Neuronal tropomodulin antibody
      • NTMOD antibody
      • TMOD2 antibody
      • tropomodulin 2 (neuronal) antibody
      • tropomodulin 2 neuronal antibody
      see all

    图片

    • All lanes : Anti-Tropomodulin 2 antibody (ab67407) at 1/500 dilution

      Lane 1 : Tropomodulin 2 transfected 293T lysate
      Lane 2 : Non transfected 293T lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Observed band size : 39.6 kDa (why is the actual band size different from the predicted?)

    文献

    This product has been referenced in:
    • Gu QH  et al. miR-26a and miR-384-5p are required for LTP maintenance and spine enlargement. Nat Commun 6:6789 (2015). Read more (PubMed: 25858512) »
    • Hu Z  et al. miR-191 and miR-135 are required for long-lasting spine remodelling associated with synaptic long-term depression. Nat Commun 5:3263 (2014). WB, ICC/IF . Read more (PubMed: 24535612) »

    See all 3 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Murine Neuroblastoma cell line N1E-115)
    Specification
    Murine Neuroblastoma cell line N1E-115
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 0.1% Triton X100
    Blocking step
    BSA as blocking agent for 45 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 03 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (N1E-115 mouse neuroblastoma)
    Loading amount
    20 µg
    Specification
    N1E-115 mouse neuroblastoma
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Feb 25 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (hippocampus)
    Loading amount
    5 µg
    Specification
    hippocampus
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Sep 06 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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