Anti-TROP2抗体[162-46.2] (ab79976)

概述

  • 产品名称
    Anti-TROP2抗体[162-46.2]
    参阅全部 TROP2 一抗
  • 描述
    小鼠单克隆抗体[162-46.2] to TROP2
  • 经测试应用
    适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human TROP2. Human choriocarcinoma cell line BeWo.

  • 阳性对照
    • IHC-P: Human placenta tissue. ICC/IF: JEG3 and MCF7 cells.
  • 常规说明

    This antibody clone is manufactured by Abcam.

    Isotype: IgG1 (Igh-4a allotype)

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    单克隆
  • 克隆编号
    162-46.2
  • 同种型
    IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79976 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能
    May function as a growth factor receptor.
  • 组织特异性
    Placenta, pancreatic carcinoma cell lines.
  • 疾病相关
    Defects in TACSTD2 are the cause of gelatinous drop-like corneal dystrophy (GDLD) [MIM:204870]; also known as lattice corneal dystrophy type III. GDLD is an autosomal recessive disorder characterized by grayish corneal amyloid deposits that cause severe visual impairment.
  • 序列相似性
    Belongs to the EPCAM family.
    Contains 1 thyroglobulin type-1 domain.
  • 翻译后修饰
    The N-terminus is blocked.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell surface glycoprotein Trop 2 antibody
    • Cell surface glycoprotein Trop-2 antibody
    • Cell surface glycoprotein Trop2 antibody
    • Epithelial glycoprotein 1 antibody
    • GA733 1 antibody
    • GA7331 antibody
    • M1S 1 antibody
    • M1S1 antibody
    • Membrane component chromosome 1 surface marker 1 antibody
    • Pancreatic carcinoma marker protein GA733 1 antibody
    • Pancreatic carcinoma marker protein GA733-1 antibody
    • Pancreatic carcinoma marker protein GA7331 antibody
    • TACD 2 antibody
    • TACD2_HUMAN antibody
    • TACSTD 2 antibody
    • Tacstd2 antibody
    • Trop 2 antibody
    • Trop2 antibody
    • Tumor associated calcium signal transducer 2 precursor antibody
    • Tumor-associated calcium signal transducer 2 antibody
    see all

Anti-TROP2 antibody [162-46.2] 图像

  • MCF7 cells stained for TROP2 (colored green) using ab79976 in ICC/IF. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing MCF7 cells stained with ab79976 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79976, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed MCF7 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • ab79976 staining TROP2 in human 10 week placenta by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Anti-mouse peroxidase IgG used as secondary antibody. Note staining of cytotrophoblast (CT) and syncytiotrophoblast (ST).

Anti-TROP2 antibody [162-46.2] (ab79976)参考文献

This product has been referenced in:
  • Viswanatha R  et al. Interactome analysis reveals ezrin can adopt multiple conformational states. J Biol Chem 288:35437-51 (2013). ICC/IF ; Human . Read more (PubMed: 24151071) »
  • Lipinski M  et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc Natl Acad Sci U S A 78:5147-50 (1981). Read more (PubMed: 7029529) »

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

Thank you for pointing out the error in figure legends for ab824 and ab79976. The flow cytometry figure legends for the two antibodies have been switched on the online datasheet to correspond with the correct an...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA/Tris
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Apr 23 2012

Vielen Dank für Ihre Anrufe.

Wie versprochen habe ich alle unsere Langerin- bzw. TACD2-Antikörper danach geprüft, ob sie schon einmal mit Schwein getestet wurden - was leider bei keinem der Fall ist.

Daher habe ich di...

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Glad to hear it! Please let me know if you need any further assistance.

Thank you for sending the data as well as all the protocol description and for explaining the experiments.

Could you please let us know if you have tried both lots (GR41084-1 and GR41084-2) and with the same results of no staining?

...

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Thank you for contacting us. I am sorry to hear that the new lot you have received is not producing good results.

After checking our records, we have not had any other complaints about this antibody in recent years. It is possible that there ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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