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Our Abpromise guarantee covers the use of ab33655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 19923466|
|IHC-P||Use at an assay dependent concentration. PubMed: 22646479|
|IP||Use at an assay dependent concentration. PubMed: 23729812|
|WB||Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).|
Immunohistochemical analysis of paraffin embedded mouse P29-RA lung section (10µm thick) labelling TrkB with ab33655 at 1:200 dilution. The secondary antibody was a peroxidase-labeled, polymer-conjugated goat anti-rabbit secondary antibody. Sections were stained with hematoxylin and eosin (H&E). Red arrow indicates bronchiolar epithelial cells, green arrow indicates type II pneumocyte and blue arrow indicates endothelial cells.