概述

  • 产品名称
  • 描述
    兔多克隆抗体to TrkB
  • 经测试应用
    适用于: ICC/IF, IHC-P, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    The entire extracellular domain (corresponding to residues 1 to 429) of rat TrkB.

  • 阳性对照
    • Mouse brain membrane protein preparation.

性能

应用

Our Abpromise guarantee covers the use of ab33655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19923466
IHC-P Use at an assay dependent concentration. PubMed: 22646479
IP Use at an assay dependent concentration. PubMed: 23729812
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 92 kDa).

靶标

  • 功能
    Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • 组织特异性
    Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Ligand-mediated auto-phosphorylation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic receptor tyrosine kinase 2 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

图片

  • Anti-TrkB antibody (ab33655) at 1 µg/ml + Mouse brain membrane protein

    Predicted band size : 92 kDa
  • Immunohistochemical analysis of paraffin embedded mouse P29-RA lung section (10µm thick) labelling TrkB with ab33655 at 1:200 dilution. The secondary antibody was a peroxidase-labeled, polymer-conjugated goat anti-rabbit secondary antibody. Sections were stained with hematoxylin and eosin (H&E). Red arrow indicates bronchiolar epithelial cells, green arrow indicates type II pneumocyte and blue arrow indicates endothelial cells.

文献

This product has been referenced in:
  • Tan Y  et al. 7,8-Dihydroxyflavone Ameliorates Cognitive Impairment by Inhibiting Expression of Tau Pathology in ApoE-Knockout Mice. Front Aging Neurosci 8:287 (2016). WB ; Mouse . Read more (PubMed: 27965573) »
  • Queiroga CS  et al. Paracrine effect of carbon monoxide - astrocytes promote neuroprotection through purinergic signaling in mice. J Cell Sci 129:3178-88 (2016). WB ; Mouse . Read more (PubMed: 27383770) »

See all 9 Publications for this product

客户评价及客户问答

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain - Cerebellar granule cells - DIV3)
Loading amount
30 µg
Specification
Brain - Cerebellar granule cells - DIV3
Treatment
100 ng/ml human BDNF for 6 minutes
Gel Running Conditions
Reduced Denaturing (8% SDS PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Mar 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (Brain - Cerebellar granule cells - DIV3)
Total protein in input
700 µg
Specification
Brain - Cerebellar granule cells - DIV3
Treatment
100 ng/ml human BDNF for 6 minutes
Immuno-precipitation step
Protein A
Username

Abcam user community

Verified customer

提交于 Mar 12 2013

Thank you for contacting us. Having compared the anti-phospho antibodies provided by Santa Cruz to those of Abcam (ab52191 and ab51187) they appear to be suitable for the same application in targeting the phosphorylated tyrosine-705 and -515 of...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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