概述

  • 产品名称Anti-TrkB抗体
    参阅全部 TrkB 一抗
  • 描述
    兔多克隆抗体to TrkB
  • 特异性ab18987 recognizes trkB from samples of human and rat origins. Trk protein exists as variably glycosylated entities with the major forms having molecular weights of 140 kDa, 110 kDa, and the unglycosylated form of 80 kDa.
  • 经测试应用适用于: ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to Human TrkB. Surrounding amino acid 810 of human TrkB.
    (Peptide available as ab52216)

  • 阳性对照
    • HL-60 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab18987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).Can be blocked with TrkB peptide (ab52216).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • 组织特异性Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰Ligand-mediated auto-phosphorylation.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

Anti-TrkB antibody 图像

  • ab18987 staining TrkB in rat PC12 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with methanol, permeabilized with 0.1% Saponin / PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (5 µg/ml in blocking buffer) for 14 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (ab150077) (1/100) was used as the secondary antibody.

    See Abreview

  • ab18987 staining TrkB in human skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.1% saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab18987 at 5µg/ml for 14 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at 15µg/ml.

    See Abreview

  • Anti-TrkB antibody (ab18987) + HL-60 Cell Lysate.

    Predicted band size : 92 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)

Anti-TrkB antibody (ab18987)参考文献

This product has been referenced in:
  • Zhong L  et al. Propofol exposure during late stages of pregnancy impairs learning and memory in rat offspring via the BDNF-TrkB signalling pathway. J Cell Mol Med 20:1920-31 (2016). Rat . Read more (PubMed: 27297627) »
  • Zhang HH  et al. The BDNF/TrkB signaling pathway is involved in heat hyperalgesia mediated by Cdk5 in rats. PLoS One 9:e85536 (2014). WB ; Rat . Read more (PubMed: 24465591) »

See all 5 Publications for this product

Product Wall

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 15 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Whole Brain Sections)
Specification Whole Brain Sections
Permeabilization Yes - 10% Tween-20
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 16 2014

Thank you for contacting us. We recommend using ab18987 with a concentration of at least 2ug/mL for immunocytochemistry on cultured cells. Given the 0.2mg/mL stock concentration for this antibody, that would be a 1:100 dilution. For the strongest signa...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (PC12 cells (neuronal cells))
Loading amount 20 µg
Specification PC12 cells (neuronal cells)
Treatment see below (Additional notes)
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (PC12 cells (neuronal cells))
Specification PC12 cells (neuronal cells)
Fixative Methanol
Permeabilization Yes - 0,1% Saponin / PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization Yes - 0,1% Saponin / PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mousse Cortical neurons and rat brain)
Loading amount 10 µg
Specification Mousse Cortical neurons and rat brain
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Carles Gil

Verified customer

提交于 Jun 03 2011

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (sebocytes)
Loading amount 100 µg
Specification sebocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% TBST
Username

Abcam user community

Verified customer

提交于 Jul 21 2009

Thank you for your enquiry. I can confirm that the immunogen sequence used to generate this antibody was from sequence reference S76473.1 which is the 90kda form and that this 90kda form has been successfully detected with the antibody. I would pre...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"