概述

  • 产品名称
  • 描述
    兔多克隆抗体to TrkB
  • 特异性
    ab18987 recognizes trkB from samples of human and rat origins. Trk protein exists as variably glycosylated entities with the major forms having molecular weights of 140 kDa, 110 kDa, and the unglycosylated form of 80 kDa.
  • 经测试应用
    适用于: ICC/IF, IP, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide corresponding to Human TrkB. Surrounding amino acid 810 of human TrkB.
    (Peptide available as ab52216)

  • 阳性对照
    • HL-60 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab18987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 - 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).Can be blocked with TrkB peptide (ab52216).
IHC-P Use at an assay dependent concentration.

靶标

  • 功能
    Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
  • 组织特异性
    Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
  • 序列相似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Ligand-mediated auto-phosphorylation.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI848316 antibody
    • BDNF tropomyosine receptor kinase B antibody
    • BDNF/NT 3 growth factors receptor antibody
    • BDNF/NT-3 growth factors receptor antibody
    • Brain derived neurotrophic factor receptor antibody
    • C030027L06Rik antibody
    • EC 2.7.10.1 antibody
    • GP145 TrkB antibody
    • GP145-TrkB antibody
    • GP145-TrkB/GP95-TrkB antibody
    • GP95 TrkB antibody
    • Neurotrophic receptor tyrosine kinase 2 antibody
    • Neurotrophic tyrosine kinase receptor type 2 antibody
    • Neurotrophin receptor tyrosine kinase type 2 antibody
    • NTRK 2 antibody
    • Ntrk2 antibody
    • NTRK2_HUMAN antibody
    • Obesity, hyperphagia, and developmental delay, included antibody
    • RATTRKB1 antibody
    • Tkrb antibody
    • Trk B antibody
    • Trk-B antibody
    • TRKB antibody
    • TrkB tyrosine kinase antibody
    • TRKB1 antibody
    • Tropomyosin related kinase B antibody
    • tyrosine kinase receptor B antibody
    • Tyrosine receptor kinase B antibody
    see all

图片

  • Anti-TrkB antibody (ab18987) + HL-60 Cell Lysate.

    Predicted band size : 92 kDa
    Observed band size : >90 kDa (why is the actual band size different from the predicted?)
  • ab18987 staining TrkB in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/250 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • ab18987 staining TrkB in human skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer pH 6.0. Samples were then permeabilized using 0.1% saponin/PBS, blocked with 4% serum for 30 minutes at 25°C and then incubated with ab18987 at 5µg/ml for 14 hours at 4°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at 15µg/ml.

    See Abreview

  • ab18987 staining TrkB in rat PC12 cells by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with methanol, permeabilized with 0.1% Saponin / PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (5 µg/ml in blocking buffer) for 14 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (ab150077) (1/100) was used as the secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Wang TY  et al. Expression profiles and function analysis of microRNAs in postovulatory aging mouse oocytes. Aging (Albany NY) 9:1186-1201 (2017). WB ; Mouse . Read more (PubMed: 28394765) »
  • Jiang D  et al. miR-124 promotes the neuronal differentiation of mouse inner ear neural stem cells. Int J Mol Med 38:1367-1376 (2016). WB . Read more (PubMed: 28025992) »

See all 11 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X-100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Spinal cord
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

提交于 Feb 13 2017

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Aug 15 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Heat-Inactivated Normal Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Whole Brain Sections)
Specification
Whole Brain Sections
Permeabilization
Yes - 10% Tween-20
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Dec 16 2014

Thank you for contacting us. We recommend using ab18987 with a concentration of at least 2ug/mL for immunocytochemistry on cultured cells. Given the 0.2mg/mL stock concentration for this antibody, that would be a 1:100 dilution. For the strongest signa...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12 cells (neuronal cells))
Loading amount
20 µg
Specification
PC12 cells (neuronal cells)
Treatment
see below (Additional notes)
Gel Running Conditions
Reduced Non-Denaturing (Native) (5-16% gels)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (PC12 cells (neuronal cells))
Specification
PC12 cells (neuronal cells)
Fixative
Methanol
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Dec 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mousse Cortical neurons and rat brain)
Loading amount
10 µg
Specification
Mousse Cortical neurons and rat brain
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Carles Gil

Verified customer

提交于 Jun 03 2011

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (sebocytes)
Loading amount
100 µg
Specification
sebocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% TBST
Username

Abcam user community

Verified customer

提交于 Jul 21 2009

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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