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Synthetic peptide corresponding to Human TrkB. Surrounding amino acid 810 of human TrkB.
(Peptide available as
Our Abpromise guarantee covers the use of ab18987 in the following tested applications.
|ICC/IF||Use a concentration of 2 - 10 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).Can be blocked with TrkB peptide (ab52216).|
|IHC-P||Use at an assay dependent concentration.|
ab18987 staining TrkB in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/250 in blocking buffer) for 2 hours at 21°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.
ab18987 staining TrkB in rat PC12 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with methanol, permeabilized with 0.1% Saponin / PBS and blocked with 4% serum for 30 minutes at 25°C. Samples were incubated with primary antibody (5 µg/ml in blocking buffer) for 14 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (ab150077) (1/100) was used as the secondary antibody.