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Extracellular fragment from the rat Trk A receptor (amino acids 1-416)
Our Abpromise guarantee covers the use of ab8871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||1/1000 - 1/10000.|
|IP||Use at an assay dependent concentration. Use at a concentration of 2 - 5 µg/30µl Protein A sepharose per sample. Incubate for 2-3 hours at 4°C, then wash the Sepharose beads four times with lysis buffer. Elute sepharose-bound proteins in 2x Laemmli Sample buffer.|
|WB||Use a concentration of 1 µg/ml.|
|ICC||Use at an assay dependent concentration.|
Image courtesy of an Abreview submitted by Joe Makkerh.