Anti-TRK fused gene抗体(ab86606)

概述

  • 产品名称Anti-TRK fused gene抗体
    参阅全部 TRK fused gene 一抗
  • 描述
    兔多克隆抗体to TRK fused gene
  • 经测试应用适用于: WB, IP, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to region between residues 175 and 225 of human TRK fused gene (NP_001007566.1)

  • 阳性对照
    • HeLa, 293T and NIH3T3 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab86606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 组织特异性Ubiquitous.
  • 疾病相关Defects in TFG are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TFG is found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with NTRK1. The TFG sequence is fused to the 3'-end of NTRK1 generating the TRKT3 (TRK-T3) fusion transcript.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ36137 antibody
    • HMSNP antibody
    • OTTHUMP00000214045 antibody
    • OTTHUMP00000214046 antibody
    • OTTHUMP00000214047 antibody
    • OTTHUMP00000214048 antibody
    • Protein TFG antibody
    • SPG57 antibody
    • TF6 antibody
    • TFG antibody
    • TFG_HUMAN antibody
    • TRK fused antibody
    • TRK fused gene antibody
    • TRK fused gene protein antibody
    • TRK-fused gene protein antibody
    • TRKT3 antibody
    • TRKT3 oncogene antibody
    see all

Anti-TRK fused gene antibody 图像

  • All lanes : Anti-TRK fused gene antibody (ab86606) at 1/0.1 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 44 kDa


    Exposure time : 30 seconds
  • Detection of TRK fused gene by Western Blot of Immunprecipitate.
    ab86606 at 1µg/ml staining TRK fused gene in HeLa whole cell lysate immunoprecipitated using ab86606 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.
  • ICC/IF image of ab86606 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86606, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TRK fused gene antibody (ab86606)参考文献

ab86606 has not yet been referenced specifically in any publications.

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