Anti-Tristetraprolin抗体(ab83579)

概述

  • 产品名称Anti-Tristetraprolin抗体
    参阅全部 Tristetraprolin 一抗
  • 描述
    兔多克隆抗体to Tristetraprolin
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Tristetraprolin.

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; HepG2; Jurkat; Ramos; A431; SK N SH; PC3.

性能

应用

Our Abpromise guarantee covers the use of ab83579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).
  • 序列相似性Contains 2 C3H1-type zinc fingers.
  • 翻译后修饰Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.
  • 细胞定位Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G0/G1 switch regulatory protein 24 antibody
    • G0S24 antibody
    • GOS24 antibody
    • Growth factor-inducible nuclear protein NUP475 antibody
    • NUP475 antibody
    • Protein TIS11A antibody
    • RNF162A antibody
    • TIS 11 antibody
    • TIS11 antibody
    • TIS11A antibody
    • Tristetraprolin antibody
    • Tristetraproline antibody
    • TTP antibody
    • TTP_HUMAN antibody
    • Zfp-36 antibody
    • ZFP36 antibody
    • Zinc finger protein 36 antibody
    • Zinc finger protein 36 homolog antibody
    • Zinc finger protein 36, C3H type, homolog (mouse) antibody
    • Zinc finger protein, C3H type, 36 homolog antibody
    see all

Anti-Tristetraprolin antibody 图像

  • All lanes : Anti-Tristetraprolin antibody (ab83579) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 5 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 6 : SK N SH (Human neuroblastoma) Whole Cell Lysate
    Lane 7 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 150 kDa,25 kDa,70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    Tristetraprolin contains a number of potential phosphorylation sites (SwissProt data) which may explain it running at a higher molecular weight than predicted. The expression profile observed is also consistent with what has been described in the literature (PMID:16508015).

  • Anti-Tristetraprolin antibody (ab83579) at 1/1000 dilution + HeLa whole cell lysate at 20 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 44 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-Tristetraprolin antibody (ab83579)参考文献

ab83579 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Cell lysate - whole cell (Hela)
Specification Hela
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Sep 18 2014

Thank you for your reply.

I have heard back from the lab regarding ab83579:


Crossreactivity with TISIIB and TISIID isn't something we have tested in house but an immunogen search shows that both TISIIB and TISIID only have 4 resi...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

提交于 Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"