Anti-Tristetraprolin抗体[8B5] (ab124024)

概述

  • 产品名称Anti-Tristetraprolin抗体[8B5]
    参阅全部 Tristetraprolin 一抗
  • 描述
    小鼠单克隆抗体[8B5] to Tristetraprolin
  • 经测试应用适用于: IHC-P, Flow Cyt, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length Human Tristetraprolin produced in HEK293T cells (NP_003398).

  • 阳性对照
    • HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin cDNA; Human colon, colon adenocarcinoma, ovary, endometrium and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY Tristetraprolin.
  • 常规说明Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

性能

应用

Our Abpromise guarantee covers the use of ab124024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.
WB 1/1 - 1/1000.

靶标

  • 功能mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).
  • 序列相似性Contains 2 C3H1-type zinc fingers.
  • 翻译后修饰Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.
  • 细胞定位Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G0/G1 switch regulatory protein 24 antibody
    • G0S24 antibody
    • GOS24 antibody
    • Growth factor-inducible nuclear protein NUP475 antibody
    • NUP475 antibody
    • Protein TIS11A antibody
    • RNF162A antibody
    • TIS 11 antibody
    • TIS11 antibody
    • TIS11A antibody
    • Tristetraprolin antibody
    • Tristetraproline antibody
    • TTP antibody
    • TTP_HUMAN antibody
    • Zfp-36 antibody
    • ZFP36 antibody
    • Zinc finger protein 36 antibody
    • Zinc finger protein 36 homolog antibody
    • Zinc finger protein 36, C3H type, homolog (mouse) antibody
    • Zinc finger protein, C3H type, 36 homolog antibody
    see all

Anti-Tristetraprolin antibody [8B5] 图像

  • All lanes : Anti-Tristetraprolin antibody [8B5] (ab124024)

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin cDNA

    Lysates/proteins at 5 µg per lane.
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202049)
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human colon tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human ovary tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human endometrium tissue by Immunohistochemistry.
  • ab124024, at 1/50, staining Tristetraprolin in paraffin embedded Human prostate tissue by Immunohistochemistry.
  • ab124024, at 1/100, staining Tristetraprolin in COS7 cells transiently transfected with pCMV6-ENTRY Tristetraprolin by Immunofluorescence.
  • ab124024 at 1/100 dilution staining Tristetraprolin in HEK293T cells transfected with either pCMV6-ENTRY Tristetraprolin (Red) or empty vector control plasmid (Blue) by flow cytometry.

Anti-Tristetraprolin antibody [8B5] (ab124024)参考文献

ab124024 has not yet been referenced specifically in any publications.

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Thank you for providing that information and for your patience and understanding in this matter.

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Unfortunately that PO# does not match the one that we have in our system. Is this the PO# that Cedalane told you they provided to Abcam, or is this the PO# that you provided to Cedarlane?

Before I can proce...

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It will not be an issue replacing the antibody even though it was purchased through Cedarlane. What I do need though is the PO# that Cedarlane supplied to Abcam and the date they placed the order. If you could contac...

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As the antibody is not giving you satisfactory results in human samples, I will be more than happy to replace or refund the antibody for you. We do not have any antibodies aga...

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The antibody ab124024 is covered under our Abpromise for six months and is guaranteed to work in western blot on human samples. We have not tested the antibody on mouse samples and you mention in your original ...

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We have 1 mouse monoclonal antibody available for which ICC/IF is a tested application. If this antibodies does detect ZFP36 in negative cells we will offer you refund.

http://www.abcam.com/Tristetraproli...

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The details provided will enable us to investigate this case and will provide us with vita...

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I am sorry to hear that this product is not performing as expected. This product is covered by our Abpromise guarantee. If this is used according to the specification listed on the datasheet (on human in IHC-P,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"