Triptolide from Tripterygium wilfordii,Anti-inflammatory agent (ab120720)
Key features and details
- Anti-inflammatory agent with multiple biological activities
- CAS Number: 38748-32-2
- Purity: > 99%
- Soluble in DMSO to 100 mM
- Form / State: Solid
- Source: Tripterygium wilfordii
概述
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产品名称
Triptolide from Tripterygium wilfordii,Anti-inflammatory agent -
描述
Anti-inflammatory agent with multiple biological activities -
别名
- PG490
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生物学描述
Active ingredient of the vineTripterygium wilfodii Hook. f. Anti-inflammatory and immunosuppressive agent. Inhibits NF-κB transcriptional activity. Additionally, shows antiproliferative and pro-apoptotic activites. Downregulates RAC1, JAK/STAT 3 pathway and has anticancer properties.
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纯度
> 99% -
CAS编号
38748-32-2 -
化学结构
性能
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化学名称
(3bS,4aS,5aS,6R,6aR,7aS,7bS,8aS,8bS)-3b,4,4a,6,6a,7a,7b,8b,9,10-Decahydro-6-hydroxy-8b-methyl-6a-(1-methylethyl)trisoxireno[4b,5:6,7:8a,9]phenanthro[1,2-c]furan-1(3H)-one -
分子量
360.41 -
分子式
C20H24O6 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in DMSO to 100 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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来源
Tripterygium wilfordii
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab120720于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Functional Studies |
Use at an assay dependent concentration.
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说明 |
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Functional Studies
Use at an assay dependent concentration. |
图片
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2D chemical structure image of ab120720, Triptolide from Tripterygium wilfordii, Anti-inflammatory agent
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ab22758 staining nucleolin in HeLa cells treated with Triptolide from Tripterygium wilfordii (ab120720), by ICC/IF. Changes in nuclear localization of nucleolin correlates with increased concentration of Triptolide from Tripterygium wilfordii, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120720 (Triptolide from Tripterygium wilfordii ) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab22758 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab120720 被引用在 2 文献中.
- Gillen SL et al. Differential regulation of mRNA fate by the human Ccr4-Not complex is driven by coding sequence composition and mRNA localization. Genome Biol 22:284 (2021). PubMed: 34615539
- Zhang H et al. DEAD-Box Helicase 18 Counteracts PRC2 to Safeguard Ribosomal DNA in Pluripotency Regulation. Cell Rep 30:81-97.e7 (2020). PubMed: 31914400