The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Use at 2-5 µg/mg of lysate.
功能Relays signals from the cell surface to the nucleus to weaken adherens junction and promote actin cytoskeleton reorganization and cell invasiveness. Involved in lysophosphatidic acid-induced cell adhesion and migration. Acts as a transcriptional coactivator for NF-kappa-B and JUN, and mediates the transrepression of these transcription factors induced by glucocorticoid receptor.
组织特异性Abundantly expressed in kidney, liver and lung. Lower levels in heart, placenta and pancreas. Expressed in colonic epithelial cells. Up-regulated in colonic tumors.
序列相似性Belongs to the zyxin/ajuba family. Contains 3 LIM zinc-binding domains.
结构域The LIM zinc-binding domains mediate interaction with LPAR2 and with S.typhimurium protein sseI.
翻译后修饰Phosphorylation at Tyr-55 by SRC is required for enhancement of lysophosphatidic acid-induced cell migration. Tyr-55 is dephosphorylated by PTPN13.
细胞定位Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
1mg whole cell lysate from HeLa cells was immunprecipitated using ab70747 at 3µg/mg of lysate. For the subsequent western blot, 20% of the immunoprecipitate was probed with ab70747 at 1ug/ml. Detection: chemiluminescence with exposure time of 10 seconds.
Anti-TRIP6 antibody (ab70747)参考文献
has not yet been referenced specifically in any publications.