概述

  • 产品名称Anti-TRIP230抗体
  • 描述
    兔多克隆抗体to TRIP230
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    A region between residues 25 and 75 of TRIP230

  • 阳性对照
    • Whole cell lysates from HeLa and 293T cells.

性能

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应用

Our Abpromise guarantee covers the use of ab72223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 228 kDa).
IP Use at 2-5 µg/mg of lysate.

靶标

  • 相关性TRIP230 was identified through its ability to interact functionally with thyroid hormone receptor-beta. It has also been found in association with the Golgi apparatus and microtubules. TRIP230 binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. This protein is probably involved in maintaining cis-Golgi structure.
  • 细胞定位Cell Membrane and Golgi Apparatus
  • 数据库链接
  • 别名
    • CEV 14 antibody
    • CEV14 antibody
    • Clonal evolution related gene on chromosome 14 antibody
    • GMAP 210 antibody
    • GMAP210 antibody
    • Golgi associated microtubule binding protein 210 antibody
    • Thyroid hormone receptor interactor 11 antibody
    • Thyroid receptor interacting protein 11 antibody
    • TRIP 11 antibody
    • TRIP 230 antibody
    • TRIP11 antibody
    see all

Anti-TRIP230 antibody 图像

  • All lanes : Anti-TRIP230 antibody (ab72223) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 228 kDa
    Observed band size : 238 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 seconds
  • Detection of Human TRIP230 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with ab72223 at 3µg in lane 1, an antibody recognising a downstream epitope in lane 2 and control IgG in lane 3. Image shows immunoprecipitated TRIP230 detected with post IP WB, loading 20% of IP and using ab72223 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Anti-TRIP230 antibody (ab72223)参考文献

ab72223 has not yet been referenced specifically in any publications.

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