Anti-TRIP13抗体- Carboxyterminal end (ab64964)

概述

  • 产品名称
    Anti-TRIP13抗体- Carboxyterminal end
    参阅全部 TRIP13 一抗
  • 描述
    兔多克隆抗体to TRIP13 - Carboxyterminal end
  • 特异性
    Detects endogenous levels of total TRIP13 protein.
  • 经测试应用
    适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from C terminal of human TRIP13.

  • 阳性对照
    • Extracts from LOVO cells and NIH 3T3 cells IHC-P: Human lunf FFPE tissue sections.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

靶标

  • 序列相似性
    Belongs to the AAA ATPase family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 16E1-BP antibody
    • 16E1BP antibody
    • Homo sapiens HPV16 E1 protein binding protein mRNA complete cds antibody
    • HPV16 E1 protein binding protein antibody
    • HPV16 E1 protein-binding protein antibody
    • Human papillomavirus type 16 E1 protein binding protein antibody
    • Human papillomavirus type 16 E1 protein-binding protein antibody
    • Pachytene checkpoint protein 2 homolog antibody
    • PCH2 antibody
    • Thyroid hormone receptor interactor 13 antibody
    • Thyroid receptor interacting protein 13 antibody
    • Thyroid receptor-interacting protein 13 antibody
    • TR-interacting protein 13 antibody
    • TRIP-13 antibody
    • Trip13 antibody
    • TRP13_HUMAN antibody
    see all

Anti-TRIP13 antibody - Carboxyterminal end 图像

  • IHC image of TRIP13 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TRIP13 antibody - Carboxyterminal end (ab64964) at 1/500 dilution

    Lane 1 : extracts from LOVO cells without immunizing peptide
    Lane 2 : extract from NIH 3T3 cells, without immunizing peptide
    Lane 3 : extract from NIH 3T3 cells with immunizing peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

Anti-TRIP13 antibody - Carboxyterminal end (ab64964)参考文献

This product has been referenced in:
  • Pressly JD  et al. TRIP13-deficient tubular epithelial cells are susceptible to apoptosis following acute kidney injury. Sci Rep 7:43196 (2017). Read more (PubMed: 28256593) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - whole cell (NIH 3T3)
Specification
NIH 3T3
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Aug 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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