Anti-TRIP13抗体- Carboxyterminal end (ab64964)

概述

  • 产品名称Anti-TRIP13抗体- Carboxyterminal end
    参阅全部 TRIP13 一抗
  • 描述
    兔多克隆抗体to TRIP13 - Carboxyterminal end
  • 特异性Detects endogenous levels of total TRIP13 protein.
  • 经测试应用适用于: IHC-P, ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from C terminal of human TRIP13.

  • 阳性对照
    • Extracts from LOVO cells and NIH 3T3 cells IHC-P: Human lunf FFPE tissue sections.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab64964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

靶标

  • 序列相似性Belongs to the AAA ATPase family.
  • Information by UniProt
  • 数据库链接
  • 别名
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    • 16E1BP antibody
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    • HPV16 E1 protein-binding protein antibody
    • Human papillomavirus type 16 E1 protein binding protein antibody
    • Human papillomavirus type 16 E1 protein-binding protein antibody
    • Pachytene checkpoint protein 2 homolog antibody
    • PCH2 antibody
    • Thyroid hormone receptor interactor 13 antibody
    • Thyroid receptor interacting protein 13 antibody
    • Thyroid receptor-interacting protein 13 antibody
    • TR-interacting protein 13 antibody
    • TRIP-13 antibody
    • Trip13 antibody
    • TRP13_HUMAN antibody
    see all

Anti-TRIP13 antibody - Carboxyterminal end 图像

  • IHC image of TRIP13 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TRIP13 antibody - Carboxyterminal end (ab64964) at 1/500 dilution

    Lane 1 : extracts from LOVO cells without immunizing peptide
    Lane 2 : extract from NIH 3T3 cells, without immunizing peptide
    Lane 3 : extract from NIH 3T3 cells with immunizing peptide


    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

Anti-TRIP13 antibody - Carboxyterminal end (ab64964)参考文献

ab64964 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (12% SDS-PAGE)
Sample Human Cell lysate - whole cell (NIH 3T3)
Specification NIH 3T3
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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提交于 Aug 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"